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原子力显微镜作为神经科学中的一种先进工具。

Atomic force microscopy as an advanced tool in neuroscience.

作者信息

Jembrek Maja Jazvinšćak, Šimić Goran, Hof Patrick R, Šegota Suzana

机构信息

Division of Molecular Medicine, Ruđer Bošković Institute, POB 180, Zagreb, Croatia.

Department for Neuroscience, Croatian Institute for Brain Research, University of Zagreb Medical School, Zagreb, Croatia.

出版信息

Transl Neurosci. 2015 Jun 11;6(1):117-130. doi: 10.1515/tnsci-2015-0011. eCollection 2015.

DOI:10.1515/tnsci-2015-0011
PMID:28123795
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4936619/
Abstract

This review highlights relevant issues about applications and improvements of atomic force microscopy (AFM) toward a better understanding of neurodegenerative changes at the molecular level with the hope of contributing to the development of effective therapeutic strategies for neurodegenerative illnesses. The basic principles of AFM are briefly discussed in terms of evaluation of experimental data, including the newest PeakForce Quantitative Nanomechanical Mapping (QNM) and the evaluation of Young's modulus as the crucial elasticity parameter. AFM topography, revealed in imaging mode, can be used to monitor changes in live neurons over time, representing a valuable tool for high-resolution detection and monitoring of neuronal morphology. The mechanical properties of living cells can be quantified by force spectroscopy as well as by new AFM. A variety of applications are described, and their relevance for specific research areas discussed. In addition, imaging as well as non-imaging modes can provide specific information, not only about the structural and mechanical properties of neuronal membranes, but also on the cytoplasm, cell nucleus, and particularly cytoskeletal components. Moreover, new AFM is able to provide detailed insight into physical structure and biochemical interactions in both physiological and pathophysiological conditions.

摘要

本综述重点介绍了原子力显微镜（AFM）在应用和改进方面的相关问题，旨在更好地从分子水平理解神经退行性变化，以期为神经退行性疾病有效治疗策略的发展做出贡献。从实验数据评估的角度简要讨论了AFM的基本原理，包括最新的峰值力定量纳米力学映射（QNM）以及作为关键弹性参数的杨氏模量评估。成像模式下显示的AFM形貌可用于监测活神经元随时间的变化，是高分辨率检测和监测神经元形态的宝贵工具。活细胞的力学特性可通过力谱以及新型AFM进行量化。文中描述了多种应用，并讨论了它们与特定研究领域的相关性。此外，成像模式和非成像模式不仅可以提供有关神经元膜的结构和力学特性的特定信息，还能提供有关细胞质、细胞核，特别是细胞骨架成分的信息。而且，新型AFM能够深入了解生理和病理生理条件下的物理结构和生化相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/fbe9c125f3e6/tnsci-2015-0011f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/da9b8142d27a/tnsci-2015-0011f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/2439464500b2/tnsci-2015-0011f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/7300d0895916/tnsci-2015-0011f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/55bcb45f8eb8/tnsci-2015-0011f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/d0af18b84870/tnsci-2015-0011f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/f562914c4fb4/tnsci-2015-0011f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/fbe9c125f3e6/tnsci-2015-0011f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/da9b8142d27a/tnsci-2015-0011f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/2439464500b2/tnsci-2015-0011f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/7300d0895916/tnsci-2015-0011f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/55bcb45f8eb8/tnsci-2015-0011f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/d0af18b84870/tnsci-2015-0011f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/f562914c4fb4/tnsci-2015-0011f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c58/4936619/fbe9c125f3e6/tnsci-2015-0011f7.jpg

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