Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University) , Pine Gully Road, Wagga Wagga, NSW 2650 , Australia.
Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), School of Agricultural & Wine Sciences, Charles Sturt University , Locked Bag 588, Wagga Wagga, NSW 2650 , Australia.
Breed Sci. 2014 May;64(1):83-9. doi: 10.1270/jsbbs.64.83.
Phomopsis blight in Lupinus albus is caused by a fungal pathogen, Diaporthe toxica. It can invade all plant parts, leading to plant material becoming toxic to grazing animals, and potentially resulting in lupinosis. Identifying sources of resistance and breeding for resistance remains the best strategy for controlling Phomopsis and reducing lupinosis risks. However, loci associated with resistance to Phomopsis blight have not yet been identified. In this study, quantitative trait locus (QTL) analysis identified genomic regions associated with resistance to Phomopsis pod blight (PPB) using a linkage map of L. albus constructed previously from an F8 recombinant inbred line population derived from a cross between Kiev-Mutant (susceptible to PPB) and P27174 (resistant to PPB). Phenotyping was undertaken using a detached pod assay. In total, we identified eight QTLs for resistance to PPB on linkage group (LG) 3, LG6, LG10, LG12, LG17 and LG27 from different phenotyping environments. However, at least one QTL, QTL-5 on LG10 was consistently detected in both phenotyping environments and accounted for up to 28.2% of the total phenotypic variance. The results of this study showed that the QTL-2 on LG3 interacts epistatically with QTL-5 and QTL-6, which map on LG10 and LG12, respectively.
白羽扇豆褐斑病由真菌病原体 Diaporthe toxica 引起。它可以侵染所有植物部位，导致植物材料对放牧动物有毒，并且可能导致羽扇豆中毒。鉴定抗性来源和培育抗性仍然是控制 Phomopsis 和降低羽扇豆中毒风险的最佳策略。然而，与 Phomopsis 褐斑病抗性相关的基因座尚未被鉴定。在这项研究中，通过使用先前从 Kiev-Mutant（对 PPB 敏感）和 P27174（对 PPB 抗性）杂交衍生的 F8 重组自交系群体构建的白羽扇豆连锁图谱，进行了数量性状基因座（QTL）分析，鉴定了与 Phomopsis 荚腐病（PPB）抗性相关的基因组区域。使用离体荚果测定法进行表型分析。总共在连锁群（LG）3、LG6、LG10、LG12、LG17 和 LG27 上鉴定到了 8 个对 PPB 抗性的 QTL，这些 QTL 来自不同的表型环境。然而，至少有一个 QTL，LG10 上的 QTL-5 在两个表型环境中都被一致检测到，占总表型方差的 28.2%。这项研究的结果表明，LG3 上的 QTL-2 与分别位于 LG10 和 LG12 上的 QTL-5 和 QTL-6 发生上位性互作。
