Kihara Institute for Biological Research, Yokohama City University , 641-12 Maioka, Totsuka, Yokohama, Kanagawa 244-0813 , Japan.
NARO Kyushu Okinawa Agricultural Research Center , 496 Izumi, Chikugo, Fukuoka 833-0041 , Japan ; NARO Tohoku Agricultural Research Center , 50 Harajukuminami, Arai, Fukushima, Fukushima 960-2156 , Japan.
Breed Sci. 2014 May;64(1):90-6. doi: 10.1270/jsbbs.64.90.
Fusarium head blight (FHB), caused by Fusarium graminearum, is a serious disease of wheat (Triticum aestivum L.) associated with contamination by the mycotoxin deoxynivalenol (DON). The FHB-resistant wheat cultivar 'Sumai 3' has been used extensively around the world. The existence of variation in FHB resistance among 'Sumai 3' accessions has been discussed. In this study, genetic variation among 'Sumai 3' accessions collected from six countries were identified using SSR markers; our results demonstrate unique chromosome regions in Sumai 3-AUT and Sumai 3-JPN ('Sumai 3' accessions from Austria and Japan, respectively). Field evaluation indicated strong resistance to FHB in Sumai 3-AUT. The polymorphic rate (number of polymorphic markers/number of available markers × 100) based on a DArT array was 12.5% between the two 'Sumai 3' accessions. Genotyping for DNA markers flanking FHB-resistant quantitative trait loci (QTLs) revealed genetic variations for the QTL regions on 5AS and 2DS; however, no variation was observed for the QTL regions on 3BS and 6B. Thus, the variation in FHB resistance among 'Sumai 3' accessions in the field is due to genetic diversity.
镰刀菌穗腐病（FHB），由禾谷镰刀菌引起，是一种严重的小麦（Triticum aestivum L.）疾病，与真菌毒素脱氧雪腐镰刀菌烯醇（DON）的污染有关。抗 FHB 的小麦品种“Sumai 3”已在世界各地广泛使用。已经讨论了“Sumai 3”品种之间 FHB 抗性的变化。在这项研究中，使用 SSR 标记鉴定了来自六个国家的“Sumai 3”品种的遗传变异；我们的结果表明，Sumai 3-AUT 和 Sumai 3-JPN（分别来自奥地利和日本的“Sumai 3”品种）中存在独特的染色体区域。田间评估表明 Sumai 3-AUT 对 FHB 具有很强的抗性。基于 DArT 阵列的多态性率（多态标记数/可用标记数×100）在两个“Sumai 3”品种之间为 12.5%。侧翼 FHB 抗性数量性状位点（QTL）的 DNA 标记的基因型分析显示，5AS 和 2DS 上的 QTL 区域存在遗传变异；然而，3BS 和 6B 上的 QTL 区域没有观察到变异。因此，田间“Sumai 3”品种之间 FHB 抗性的变化是由于遗传多样性造成的。
