Hokkaido Research Organization, Agricultural Research Department, Chuo Agricultural Experiment Station , Higashi-6, Kita-15, Naganuma, Hokkaido 069-1395, Japan.
Breed Sci. 2012 Mar;62(1):11-7. doi: 10.1270/jsbbs.62.11. Epub 2012 Mar 20.
Fusarium head blight (FHB) is an important disease of wheat (Triticum aestivum L.). The aim of this study was to determine the effects of quantitative trait locus (QTL) regions for resistance to FHB and estimate their effects on reducing FHB damage to wheat in Hokkaido, northern Japan. We examined 233 F(1)-derived doubled-haploid (DH) lines from a cross between 'Kukeiharu 14' and 'Sumai 3' to determine their reaction to FHB during two seasons under field conditions. The DH lines were genotyped at five known FHB-resistance QTL regions (on chromosomes 3BS, 5AS, 6BS, 2DL and 4BS) by using SSR markers. 'Sumai 3' alleles at the QTLs at 3BS and 5AS effectively reduced FHB damage in the environment of Hokkaido, indicating that these QTLs will be useful for breeding spring wheat cultivars suitable for Hokkaido. Some of the QTL regions influenced agronomic traits: 'Sumai 3' alleles at the 4BS and 5AS QTLs significantly increased stem length and spike length, that at the 2DL QTL significantly decreased grain weight, and that at the 6BS QTL significantly delayed heading, indicating pleiotropic or linkage effects between these agronomic traits and FHB resistance.
镰刀菌穗腐病（FHB）是小麦（Triticum aestivum L.）的一种重要病害。本研究旨在确定抗 FHB 的数量性状位点（QTL）区域的影响，并估计其在减少日本北海道小麦 FHB 损伤方面的效果。我们在田间条件下，对来自‘Kukeiharu 14’和‘Sumai 3’杂交的 233 个 F1 衍生的双单倍体（DH）系进行了检查，以确定它们在两个季节对 FHB 的反应。DH 系通过 SSR 标记在五个已知的 FHB 抗性 QTL 区域（3BS、5AS、6BS、2DL 和 4BS 染色体上）进行了基因型分析。在北海道的环境中，3BS 和 5AS 上的‘Sumai 3’等位基因有效地降低了 FHB 损伤，表明这些 QTL 将有助于培育适合北海道的春小麦品种。一些 QTL 区域影响了农艺性状：4BS 和 5AS QTL 上的‘Sumai 3’等位基因显著增加了茎长和穗长，2DL QTL 上的等位基因显著降低了粒重，6BS QTL 上的等位基因显著延迟了抽穗，表明这些农艺性状和 FHB 抗性之间存在多效性或连锁效应。
