二十碳五烯酸和二十二碳六烯酸对Caco-2细胞中乳糜微粒和极低密度脂蛋白合成及分泌的影响。

Effects of eicosapentaenoic acid and docosahexaenoic acid on chylomicron and VLDL synthesis and secretion in Caco-2 cells.

作者信息

Wang Yue, Lin Qiaowei, Zheng Peipei, Li Lulu, Bao Zhengxi, Huang Feiruo

机构信息

Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

出版信息

Biomed Res Int. 2014;2014:684325. doi: 10.1155/2014/684325. Epub 2014 May 28.

The present research was undertaken to determine the effects of EPA (20 : 5 n-3) and DHA (22 : 6 n-3) on chylomicron and VLDL synthesis and secretion by Caco-2 cells. Cells were incubated for 12 to 36 h with 400 μM OA, EPA, and DHA; then 36 h was chosen for further study because EPA and DHA decreased de novo triglycerides synthesis in a longer incubation compared with OA (P < 0.01). Neither the uptake nor oxidation was different in response to the respective fatty acids (P > 0.05). Compared with OA, intercellular and secreted nascent apolipoprotein B48 and B100 were decreased by EPA and DHA (P < 0.01). Both DHA and EPA resulted in a lower secretion of chylomicron and VLDL (P < 0.01). In contrast to OA, EPA and DHA were preferentially incorporated into phospholipids instead of triacylglycerols (P < 0.01). These discoveries demonstrated that exposure of DHA and EPA reduced the secretion of chylomicron and VLDL partly by regulating the synthesis of TG and apoB.

本研究旨在确定二十碳五烯酸（EPA，20 : 5 n-3）和二十二碳六烯酸（DHA，22 : 6 n-3）对Caco-2细胞乳糜微粒和极低密度脂蛋白（VLDL）合成及分泌的影响。将细胞与400 μM油酸（OA）、EPA和DHA一起孵育12至36小时；然后选择36小时进行进一步研究，因为与OA相比，EPA和DHA在更长时间的孵育中降低了甘油三酯的从头合成（P < 0.01）。对各自脂肪酸的摄取和氧化均无差异（P > 0.05）。与OA相比，EPA和DHA使细胞内及分泌的新生载脂蛋白B48和B100减少（P < 0.01）。DHA和EPA均导致乳糜微粒和VLDL的分泌降低（P < 0.01）。与OA不同，EPA和DHA优先掺入磷脂而非三酰甘油中（P < 0.01）。这些发现表明，DHA和EPA的暴露通过调节甘油三酯（TG）和载脂蛋白B（apoB）的合成，部分降低了乳糜微粒和VLDL的分泌。