Abdallah Marwan, Benoliel Corinne, Jama Charafeddine, Drider Djamel, Dhulster Pascal, Chihib Nour-Eddine
Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), IUT A/Polytech'Lille, Université de Lille Science et Technologies Avenue Paul Langevin, F-59655 Villeneuve d'Ascq Cedex, France; Laboratoire SCIENTIS, Parc Biocitech - 102, Avenue Gaston Roussel, 93230 Romainville, France.
Laboratoire SCIENTIS, Parc Biocitech - 102, Avenue Gaston Roussel, 93230 Romainville, France.
J Food Prot. 2014 Jul;77(7):1116-26. doi: 10.4315/0362-028X.JFP-13-365.
This study investigated the effect of growth temperature changes (20, 30, and 37°C) on the adhesion behavior of Pseudomonas aeruginosa and Staphylococcus aureus to stainless steel and polycarbonate. Adhesion assays were performed under static conditions at 20°C. In addition, the validity of the thermodynamic and extended Derjaguin, Landau, Verwey, and Overbeek theories as predictive tools of bacterial adhesion were studied. The surface properties of the bacterial cells and the substrates of attachment were characterized, and atomic force microscopy was used to analyze the surface topography. The results indicated that the highest adhesion rate of P. aeruginosa and S. aureus on both surfaces was observed when the cells were grown at 37°C. The bacterial adhesion to stainless steel was found to be two times higher than to polycarbonate for both bacteria, whatever the condition used. The present study underlined that the thermodynamic and the extended Derjaguin, Landau, Verwey, and Overbeek theories were able to partially predict the empirical results of P. aeruginosa adhesion. However, these theories failed to predict the adhesion behavior of S. aureus to both surfaces when the growth temperature was changed. The results of the microbial adhesion to solvent indicated that the adhesion rate to abiotic surfaces may correlate with the hydrophobicity of bacterial surfaces. The effect of surface topography on bacterial adhesion showed that surface roughness, even on the very low nanometer scale, has a significant effect on bacterial adhesion behavior.
本研究调查了生长温度变化（20、30和37°C）对铜绿假单胞菌和金黄色葡萄球菌在不锈钢和聚碳酸酯上黏附行为的影响。黏附试验在20°C的静态条件下进行。此外，还研究了热力学理论以及扩展的Derjaguin、Landau、Verwey和Overbeek理论作为细菌黏附预测工具的有效性。对细菌细胞和黏附底物的表面性质进行了表征，并使用原子力显微镜分析表面形貌。结果表明，当细胞在37°C下生长时，观察到铜绿假单胞菌和金黄色葡萄球菌在两种表面上的最高黏附率。无论使用何种条件，两种细菌在不锈钢上的黏附力都比在聚碳酸酯上高两倍。本研究强调，热力学理论以及扩展的Derjaguin、Landau、Verwey和Overbeek理论能够部分预测铜绿假单胞菌黏附的实验结果。然而，当生长温度改变时，这些理论无法预测金黄色葡萄球菌在两种表面上的黏附行为。微生物对溶剂的黏附结果表明，对非生物表面的黏附率可能与细菌表面的疏水性相关。表面形貌对细菌黏附的影响表明，即使在非常低的纳米尺度上，表面粗糙度对细菌黏附行为也有显著影响。
