Saddique Haris, Aasim Muhammad, Khan Tariq, Khan Ajab, Ali Haroon Muhammad, Aziz Umar
Department of Biotechnology, University of Malakand, Chakdara, Pakistan.
Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC, USA.
AMB Express. 2025 May 6;15(1):71. doi: 10.1186/s13568-024-01820-2.
Dental caries and lesions are difficult to treat during cement repairs. A remarkable antimicrobial therapeutic biomaterial is needed to fight dental caries and recurrent necrotic lesions. This study used Mentha longifolia extract to synthesize Copper nanoparticles (CuNPs) with distinctive properties at room temperature (22-25 °C). These CuNPs were supplemented with cephalosporin antibiotics that act as a capping agent to explore their synergistic antibacterial potency. These nanoparticles were subjected to FTIR, XRD, UV-Vis spectrophotometry, and SEM for characterisation. These CuNPs capped with antibiotics were added to glass ionomer (GIC) cement. These GIC samples were divided into pure GIC and modified GIC samples. Antibiotic-supplemented CuNPs, conjugated with GIC, showed good effect against Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae and Pseudomonas aeruginosa as compared to conventional GIC, tested through a modified direct contact test. Among them, GIC enriched with cefotaxime-supplemented CuNPs exhibited excellent antibacterial effects, followed by Cefepime and Ceftriaxone-supplemented CuNPs, respectively. Pure GIC has the most negligible antibacterial effect. Further, the interaction of these modified GICs with the selected bacterial strains was studied using the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) approach. The results show that the modified GIC effectively inhibited biofilm formation on dental implants.
在进行补牙修复时，龋齿和牙体缺损很难治疗。因此，需要一种卓越的抗菌治疗性生物材料来对抗龋齿和复发性坏死性病变。本研究利用长叶薄荷提取物在室温（22 - 25°C）下合成了具有独特性质的铜纳米颗粒（CuNPs）。这些CuNPs添加了作为封端剂的头孢菌素抗生素，以探究其协同抗菌效力。对这些纳米颗粒进行了傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、紫外 - 可见分光光度法和扫描电子显微镜（SEM）表征。将这些用抗生素封端的CuNPs添加到玻璃离子水门汀（GIC）中。这些GIC样品分为纯GIC和改性GIC样品。通过改良的直接接触试验测试，与传统GIC相比，与GIC结合的添加抗生素的CuNPs对耐甲氧西林金黄色葡萄球菌、粪肠球菌、肺炎克雷伯菌和铜绿假单胞菌显示出良好的效果。其中，富含补充头孢噻肟的CuNPs的GIC表现出优异的抗菌效果，其次分别是补充头孢吡肟和头孢曲松的CuNPs。纯GIC的抗菌效果最不明显。此外，使用扩展的Derjaguin - Landau - Verwey - Overbeek（XDLVO）方法研究了这些改性GIC与所选细菌菌株的相互作用。结果表明，改性GIC有效抑制了牙种植体上生物膜的形成。
