Institute for Clean Energy & Advanced Materials, Southwest University, Chongqing 400715, PR China; Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies, Chongqing 400715, PR China.
Biosens Bioelectron. 2013 Dec 15;50:338-44. doi: 10.1016/j.bios.2013.06.037. Epub 2013 Jul 10.
Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3 pg mL(-1), respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring.
多种具有强致癌性和毒性的真菌毒素是食品安全的致命威胁，因此需要高度敏感和高通量的检测方法。本文探索了一种基于非缠结聚合物刷的荧光竞争免疫分析微阵列，该微阵列使用聚[(乙二醇)甲基丙烯酸酯-co-甲基丙烯酸缩水甘油酯]（POEGMA-co-GMA）作为敏感检测模板，以检测黄曲霉毒素 B1（AFB1）、赭曲霉毒素 A（OTA）和玉米赤霉烯酮（ZEN）等多种真菌毒素。由于 POEGMA-co-GMA 刷具有均匀的大蛋白负载量和高抗非特异性蛋白吸附能力，优化后的微阵列表现出三个数量级的宽动态范围和低检测限，分别为 4、4 和 3 pg mL(-1)，这比环氧功能化抗原微阵列要好得多，与传统的 ELISA 方法相当，甚至更好。这项工作提供了一种强大的高通量工具，可用于快速筛选食品质量和环境监测中的毒素。
