El-Shanawany M A, Sayed H M, Ibrahim S R M, Fayed M A A
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Drug Res (Stuttg). 2015 Jul;65(7):347-53. doi: 10.1055/s-0034-1382064. Epub 2014 Jul 3.
A new stigmasterol ester: stigmasterol tetracosanoate (3), along with 7 compounds: β-sitosterol (1), stigmasterol (2), (2S,3S,4R)-2[(2'R)-2'-(hydroxyeicosanoyl amino) octadecane-1,3,4-triol (4), apigenin (5), β-sitosterol-3-O-β-D-glucopyranoside (6), stigmasterol-3-O-β-D-glucopyranose (7), and apigenin-7-O-β-D-glucopyranoside (8) were isolated from Blepharis ciliaris aerial parts. Compounds 1, 2, and 5-7 are reported here for the first time from the plant and 4 for the first time from the family. GCMS analysis revealed the presence of 45 fatty acids, 53 hydrocarbons, and 24 sterols. The different fractions exhibited mild cytotoxic in brine shrimp assay and anti-hyperglycaemic activities. The EtOAc fraction and TME (total MeOH extract) showed weak anti-malarial activity against P. falciparum. The CHCl3 fraction gave potent -anti-inflammatory activity compared with indomethacin.
豆甾醇二十四烷酸酯（3），以及7种化合物：β-谷甾醇（1）、豆甾醇（2）、(2S,3S,4R)-2[(2'R)-2'-(羟基二十碳酰氨基)十八烷-1,3,4-三醇（4）、芹菜素（5）、β-谷甾醇-3-O-β-D-葡萄糖苷（6）、豆甾醇-3-O-β-D-葡萄糖（7）和芹菜素-7-O-β-D-葡萄糖苷（8）。化合物1、2、5 - 7首次从该植物中报道，化合物4首次从该科中报道。气相色谱 - 质谱联用分析显示存在45种脂肪酸、53种烃类和24种甾醇。不同馏分在卤虫试验中表现出轻度细胞毒性和抗高血糖活性。乙酸乙酯馏分和总甲醇提取物（TME）对恶性疟原虫显示出较弱的抗疟活性。与吲哚美辛相比，氯仿馏分具有较强的抗炎活性。
