Kozlova E V, Anisimova L A, Boronin A M
Antibiot Khimioter. 1989 Jan;34(1):24-8.
The levels and spectra of drug resistance were determined in 530 strains of P. aeruginosa isolated in hospitals of three cities of the USSR within 1979-1984. Their conjugative R plasmids were searched for and distribution of various type resistance determinants in the composition of these plasmids was investigated. The results were compared with the findings of analogous studies on clinical strains of P. aeruginosa isolated within 1976-1979. It was shown that there were a rise in the relative number of the strains resistant to kanamycin and a decrease in the occurrence of the P. aeruginosa strains resistant to streptomycin, tetracycline and sulfanilamides. The frequency of the kanamycin, carbenicillin and gentamicin resistance genes in the composition of the detected conjugative R plasmids increased. Hybridization of 32P-labeled probes containing various type antibiotic resistance determinants with strains of P. aeruginosa ML (PAO) containing conjugative R plasmids was indicative of wide spread of genes determining APH(3')II and APH(3") and determinants of classes A and C in the composition of the studied plasmids.
1979 - 1984年间，在苏联三个城市的医院中分离出530株铜绿假单胞菌，测定了它们的耐药水平和谱型。寻找它们的接合性R质粒，并研究这些质粒组成中各种类型耐药决定子的分布。将结果与1976 - 1979年间分离出的铜绿假单胞菌临床菌株的类似研究结果进行比较。结果表明，对卡那霉素耐药的菌株相对数量有所增加，而对链霉素、四环素和磺胺类耐药的铜绿假单胞菌菌株的发生率有所下降。在检测到的接合性R质粒组成中，卡那霉素、羧苄青霉素和庆大霉素耐药基因的频率增加。用含有各种类型抗生素耐药决定子的32P标记探针与含有接合性R质粒的铜绿假单胞菌ML（PAO）菌株杂交，表明在所研究的质粒组成中，决定APH(3')II和APH(3")的基因以及A类和C类决定子广泛传播。
