Biotechnology Laboratory, Department of Synthesis and Development of Industrial Processes, School of Chemical Engineering, National Technical University of Athens Athens, Greece ; Biochemical Process Engineering, Chemical Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology Luleå, Sweden.
Biotechnology Laboratory, Department of Synthesis and Development of Industrial Processes, School of Chemical Engineering, National Technical University of Athens Athens, Greece.
Front Microbiol. 2014 Jun 18;5:281. doi: 10.3389/fmicb.2014.00281. eCollection 2014.
The microbial conversion of solid cellulosic biomass to liquid biofuels may provide a renewable energy source for transportation fuels. Cellulolytic fungi represent a promising group of organisms, as they have evolved complex systems for adaptation to their natural habitat. The filamentous fungus Myceliophthora thermophila constitutes an exceptionally powerful cellulolytic microorganism that synthesizes a complete set of enzymes necessary for the breakdown of plant cell wall. The genome of this fungus has been recently sequenced and annotated, allowing systematic examination and identification of enzymes required for the degradation of lignocellulosic biomass. The genomic analysis revealed the existence of an expanded enzymatic repertoire including numerous cellulases, hemicellulases, and enzymes with auxiliary activities, covering the most of the recognized CAZy families. Most of them were predicted to possess a secretion signal and undergo through post-translational glycosylation modifications. These data offer a better understanding of activities embedded in fungal lignocellulose decomposition mechanisms and suggest that M. thermophila could be made usable as an industrial production host for cellulolytic and hemicellulolytic enzymes.
利用微生物将固体纤维素生物质转化为液体生物燃料，可为运输燃料提供可再生能源。纤维素分解真菌是一种很有前途的生物，因为它们已经进化出了适应自然栖息地的复杂系统。丝状真菌嗜热毁丝霉是一种具有强大纤维素分解能力的微生物，它能合成一套完整的酶，用于分解植物细胞壁。该真菌的基因组最近已被测序和注释，这使得对降解木质纤维素生物质所需的酶进行系统的检查和鉴定成为可能。基因组分析表明，存在一个扩展的酶谱，包括许多纤维素酶、半纤维素酶和具有辅助活性的酶，涵盖了大多数公认的 CAZy 家族。其中大多数被预测具有分泌信号，并经历翻译后糖基化修饰。这些数据使人们更好地理解了真菌木质纤维素分解机制中所包含的各种活性，并表明嗜热毁丝霉可能可被用作用于纤维素酶和半纤维素酶生产的工业生产宿主。
