College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China.
Bioresour Technol. 2013 Feb;129:302-7. doi: 10.1016/j.biortech.2012.11.064. Epub 2012 Nov 28.
The impact of pectinases in the hydrolysis of fresh, steam-exploded and ensiled hemp was investigated and the synergy between cellulases, pectinases and xylanase in the hydrolysis was evaluated. About half; 59.3% and 46.1% of pectin in the steam-exploded and ensiled hemp, respectively, could be removed by a low dosage of pectinases used. Pectinases were more efficient than xylanase in the hydrolysis of fresh and ensiled hemp whereas xylanase showed higher hydrolytic efficiency than the pectinase preparation used in the hydrolysis of steam-exploded hemp. Clear synergistic action between cellulases and xylanase could be observed in the hydrolysis of steam-exploded hemp. Supplementation of pectinase resulted in clear synergism with cellulases in the hydrolysis of all hemp substrates. Highest hydrolysis yield of steam-exploded hemp was obtained in the hydrolysis with cellulases and xylanase. In the hydrolysis of ensiled hemp, the synergistic action between cellulases and pectinases was more obvious for efficient hydrolysis.
研究了果胶酶对新鲜、蒸汽爆破和青贮麻的水解作用，并评估了纤维素酶、果胶酶和木聚糖酶在水解中的协同作用。用低剂量的果胶酶处理，分别有 59.3%和 46.1%的蒸汽爆破和青贮麻中的果胶可以被去除。果胶酶在水解新鲜和青贮麻方面比木聚糖酶更有效，而木聚糖酶在水解蒸汽爆破麻方面比所用的果胶酶制剂显示出更高的水解效率。在蒸汽爆破麻的水解中，可以观察到纤维素酶和木聚糖酶之间明显的协同作用。果胶酶的添加在所有麻类基质的水解中与纤维素酶产生明显的协同作用。在纤维素酶和木聚糖酶的水解作用下，蒸汽爆破麻的水解产率最高。在青贮麻的水解中，纤维素酶和果胶酶之间的协同作用对于有效的水解更为明显。
