He J, Mu X, Guo Z, Hao H, Zhang C, Zhao Z, Wang Q
Department of Respiratory Medicine, The Second Hospital, Dalian Medical University, Dalian, 116023, China,
Eur J Clin Microbiol Infect Dis. 2014 Dec;33(12):2223-30. doi: 10.1007/s10096-014-2182-z. Epub 2014 Jul 5.
Effective treatment of infectious diseases depends on the ability to rapidly identify the infecting bacteria and the use of sensitive antibiotics. The currently used identification assays usually take more than 72 h to perform and have a low sensitivity. Herein, we present a microbead-based microfluidic platform that is highly sensitive and rapid for bacterial detection and antibiotic sensitivity testing. The platform includes four units, one of which is used for bacterial identification and the other three are used for susceptibility testing. Our results showed that Escherichia coli O157 at a cell density range of 10(1)-10(5) CFU/μL could be detected within 30 min. Additionally, the effects of three antibiotics on E. coli O157 were evaluated within 4-8 h. Overall, this integrated microbead-based microdevice provides a sensitive, rapid, reliable, and highly effective platform for the identification of bacteria, as well as antibiotic sensitivity testing.
传染病的有效治疗取决于快速识别感染细菌的能力以及使用敏感抗生素。目前使用的鉴定检测通常需要超过72小时才能完成，且灵敏度较低。在此，我们展示了一种基于微珠的微流控平台，该平台对细菌检测和抗生素敏感性测试具有高灵敏度且速度快。该平台包括四个单元，其中一个用于细菌鉴定，另外三个用于药敏试验。我们的结果表明，在细胞密度范围为10(1)-10(5) CFU/μL的情况下，可在30分钟内检测到大肠杆菌O157。此外，在4-8小时内评估了三种抗生素对大肠杆菌O157的影响。总体而言，这种基于微珠的集成微型设备为细菌鉴定以及抗生素敏感性测试提供了一个灵敏、快速、可靠且高效的平台。
