Adamson J, Thorne D, Errington G, Fields W, Li X, Payne R, Krebs T, Dalrymple A, Fowler K, Dillon D, Xie F, Meredith C
British American Tobacco, Group R&D, Southampton SO15 8TL, UK.
R.J. Reynolds Tobacco Co., P.O. Box 1487, Winston-Salem, NC 27102, USA.
Toxicol In Vitro. 2014 Oct;28(7):1320-8. doi: 10.1016/j.tiv.2014.06.012. Epub 2014 Jul 2.
There are several whole smoke exposure systems used to assess the biological and toxicological impact of tobacco smoke in vitro. One such system is the Vitrocell® VC 10 Smoking Robot and exposure module. Using quartz crystal microbalances (QCMs) installed into the module, we were able to assess tobacco smoke particle deposition in real-time. We compared regional deposition across the module positions and doses delivered by six VC 10s in four independent laboratories: two in the UK, one in Germany and one in China. Gauge R&r analysis was applied to the total data package from the six VC 10s. As a percentage of the total, reproducibility (between all six VC 10s) and repeatability (error within an individual VC 10) accounted for 0.3% and 7.4% respectively. Thus Gauge R&r was 7.7%, less than 10% overall and considered statistically fit for purpose. The dose-responses obtained from the six machines across the four different locations demonstrated excellent agreement. There were little to no positional differences across the module at all airflows as determined by ANOVA (except for one machine and at three airflows only). These results support the on-going characterisation of the VC 10 exposure system and suitability for tobacco smoke exposure in vitro.
有几种全烟雾暴露系统用于在体外评估烟草烟雾的生物学和毒理学影响。其中一种系统是Vitrocell® VC 10吸烟机器人及暴露模块。通过将石英晶体微天平（QCM）安装到该模块中，我们能够实时评估烟草烟雾颗粒的沉积情况。我们比较了四个独立实验室中六个VC 10在不同模块位置和剂量下的区域沉积情况：两个在英国，一个在德国，一个在中国。量具R&R分析应用于来自六个VC 10的全部数据包。作为总数的百分比，再现性（六个VC 10之间）和重复性（单个VC 10内的误差）分别占0.3%和7.4%。因此量具R&R为7.7%，总体小于10%，在统计学上认为符合目的。从四个不同地点的六台机器获得的剂量反应显示出极好的一致性。通过方差分析确定，在所有气流下，模块上几乎没有位置差异（除了一台机器且仅在三个气流下有差异）。这些结果支持了VC 10暴露系统正在进行的特性描述以及其在体外烟草烟雾暴露中的适用性。
