Tiwari Kirti Kumar, Chu Chun, Couroucli Xanthi, Moorthy Bhagavatula, Lingappan Krithika
Department of Pediatrics, Section of Neonatology, Texas Children's Hospital, Baylor College of Medicine, 1102 Bates Avenue, MC: FC530.01, Houston, TX 77030, USA.
Department of Pediatrics, Section of Neonatology, Texas Children's Hospital, Baylor College of Medicine, 1102 Bates Avenue, MC: FC530.01, Houston, TX 77030, USA.
Biochem Biophys Res Commun. 2014 Aug 8;450(4):1345-50. doi: 10.1016/j.bbrc.2014.06.132. Epub 2014 Jul 2.
Caffeine is used to prevent bronchopulmonary dysplasia (BPD) in premature neonates. Hyperoxia contributes to the development of BPD, inhibits cell proliferation and decreases cell survival. The mechanisms responsible for the protective effect of caffeine in pulmonary oxygen toxicity remain largely unknown. A549 and MLE 12 pulmonary epithelial cells were exposed to hyperoxia or maintained in room air, in the presence of different concentrations (0, 0.05, 0.1 and 1mM) of caffeine. Caffeine had a differential concentration-specific effect on cell cycle progression, oxidative stress and viability, with 1mM concentration being deleterious and 0.05 mM being protective. Reactive oxygen species (ROS) generation during hyperoxia was modulated by caffeine in a similar concentration-specific manner. Caffeine at 1mM, but not at the 0.05 mM concentration decreased the G2 arrest in these cells. Taken together this study shows the novel funding that caffeine has a concentration-specific effect on cell cycle regulation, ROS generation, and cell survival in hyperoxic conditions.
咖啡因被用于预防早产新生儿的支气管肺发育不良（BPD）。高氧会导致BPD的发生，抑制细胞增殖并降低细胞存活率。咖啡因对肺部氧中毒保护作用的机制在很大程度上仍不清楚。将A549和MLE 12肺上皮细胞暴露于高氧环境或置于室内空气中，并添加不同浓度（0、0.05、0.1和1mM）的咖啡因。咖啡因对细胞周期进程、氧化应激和活力具有不同的浓度特异性影响，1mM浓度具有有害作用，而0.05mM浓度具有保护作用。高氧期间活性氧（ROS）的生成也以类似的浓度特异性方式受到咖啡因的调节。1mM的咖啡因而非0.05mM浓度的咖啡因可减少这些细胞中的G2期阻滞。综上所述，本研究表明了新的发现，即咖啡因在高氧条件下对细胞周期调节、ROS生成和细胞存活具有浓度特异性影响。
