Rakers S, Imse F, Gebert M
LG Aquatic Cell Technology, Fraunhofer Research Institution for Marine Biotechnology, Paul-Ehrlich-Str. 1-3, 23562, Lübeck, Germany,
Ecotoxicology. 2014 Oct;23(8):1582-91. doi: 10.1007/s10646-014-1279-6. Epub 2014 Jul 8.
In this study, we report the use of a real-time cell analysis (RTCA) test system, the xCELLigence(®) RTCA, as efficient tool for a fast cytotoxicity analysis and comparison of four different vertebrate cell cultures. This new dynamic real-time monitoring and impedance-based assay allows for a combined measurement of cell adhesion, spreading and proliferation. Cell cultures were obtained from mouse, rat, human and fish, all displaying a fibroblast-like phenotype. The measured impedance values could be correlated to characteristic cell culture behaviours. In parallel, relative cytotoxicity of a commonly used but due to its very good water solubility highly hazardous pesticide, copper sulfate, was evaluated under in vitro conditions through measurements of cell viability by classical end-point based assays MTT and PrestoBlue(®). Cell line responses in terms of viability as measured by these three methods were variable between the fish skin cells and cells from higher vertebrates and also between the three methods. The advantage of impedance-based measurements is mainly based on the continuous monitoring of cell responses for a broad range of different cells, including fish cells.
在本研究中，我们报告了使用实时细胞分析（RTCA）测试系统xCELLigence(®) RTCA作为一种高效工具，用于对四种不同脊椎动物细胞培养物进行快速细胞毒性分析和比较。这种新的动态实时监测和基于阻抗的检测方法能够联合测量细胞黏附、铺展和增殖。细胞培养物取自小鼠、大鼠、人类和鱼类，均呈现成纤维细胞样表型。测得的阻抗值可与特征性细胞培养行为相关联。同时，通过经典的基于终点法的MTT和PrestoBlue(®)检测细胞活力，在体外条件下评估了一种常用但因其水溶性很好而具有高危险性的农药硫酸铜的相对细胞毒性。通过这三种方法测得的细胞系活力反应在鱼皮肤细胞和高等脊椎动物细胞之间以及这三种方法之间存在差异。基于阻抗测量的优势主要在于能够对包括鱼类细胞在内的广泛不同细胞的细胞反应进行持续监测。
