Cánovas Angela, Rincón Gonzalo, Bevilacqua Claudia, Islas-Trejo Alma, Brenaut Pauline, Hovey Russell C, Boutinaud Marion, Morgenthaler Caroline, VanKlompenberg Monica K, Martin Patrice, Medrano Juan F
Department of Animal Science, University of California-Davis, One Shields Ave., Davis, 95616, CA, USA.
Institut National de la Recherche Agronomique, UMR 1313 Génétique animale et Biologie intégrative, F-78350 Jouy-en-Josas, France.
Sci Rep. 2014 Jul 8;4:5297. doi: 10.1038/srep05297.
The objective of this study was to examine five different sources of RNA, namely mammary gland tissue (MGT), milk somatic cells (SC), laser microdissected mammary epithelial cells (LCMEC), milk fat globules (MFG) and antibody-captured milk mammary epithelial cells (mMEC) to analyze the bovine mammary gland transcriptome using RNA-Sequencing. Our results provide a comparison between different sampling methods (invasive and non-invasive) to define the transcriptome of mammary gland tissue and milk cells. This information will be of value to investigators in choosing the most appropriate sampling method for different research applications to study specific physiological states during lactation. One of the simplest procedures to study the transcriptome associated with milk appears to be the isolation of total RNA directly from SC or MFG released into milk during lactation. Our results indicate that the SC and MFG transcriptome are representative of MGT and LCMEC and can be used as effective and alternative samples to study mammary gland expression without the need to perform a tissue biopsy.
本研究的目的是检测五种不同的RNA来源，即乳腺组织（MGT）、乳体细胞（SC）、激光显微切割乳腺上皮细胞（LCMEC）、乳脂肪球（MFG）和抗体捕获的乳腺上皮细胞（mMEC），以利用RNA测序分析牛乳腺转录组。我们的结果对不同采样方法（有创和无创）进行了比较，以确定乳腺组织和乳细胞的转录组。这些信息对于研究人员为不同研究应用选择最合适的采样方法以研究泌乳期间的特定生理状态具有重要价值。研究与乳汁相关转录组的最简单方法之一似乎是直接从泌乳期间释放到乳汁中的SC或MFG中分离总RNA。我们的结果表明，SC和MFG转录组代表了MGT和LCMEC，可作为研究乳腺表达的有效替代样本，而无需进行组织活检。
