Liu Yichen, Li Zhihui, Shi Xiuyu, Liu Ying, Li Weiguang, Duan Guofang, Li Hualing, Yang Xiaofan, Zhang Chenggang, Zou Liping
Department of Pediatrics, General Hospital of Chinese People's Liberation Army, Beijing, 100853, China.
Neurochem Res. 2014 Sep;39(9):1724-32. doi: 10.1007/s11064-014-1366-3. Epub 2014 Jul 8.
This study investigated the neuroprotection and potential mechanism of carbon monoxide (CO) against perinatal hypoxic-ischemic brain damage in rats by electrical acupuncture (EA). Animal behavior, morphological changes, cystathionine beta-synthase (CBS), hypoxia-inducible factor-1α (HIF-1α), and heme oxygenase-1 (HO-1) expression levels, and CO content in rat cortex cells were determined. Results demonstrated that EA treatment decreased the slope behavior and increased the overhang behavior of perinatal rats. The treatment also decreased the number of positive cells. The activator and inhibitor of CBS aggravated and remitted the hypoxic damage in cortex cells, respectively. EA treatment decreased CBS expression level and increased HO-1 and HIF-1α expression levels in perinatal rat cortex cells. Compared with the control groups, the CO content of cortex cells in the EA treatment group significantly increased (**p < 0.01). We hypothesized that EA treatment increases cortical CO content to protect against hypoxic damage via the hydrogen sulfide/CBS-CO/HO-1-HIF-1α system. This study provided a significant reference for EA therapy and cued a novel protective mechanism for cerebral palsy.
本研究通过电针（EA）探讨一氧化碳（CO）对大鼠围产期缺氧缺血性脑损伤的神经保护作用及其潜在机制。测定了动物行为、形态学变化、胱硫醚β-合酶（CBS）、缺氧诱导因子-1α（HIF-1α）和血红素加氧酶-1（HO-1）的表达水平以及大鼠皮质细胞中的CO含量。结果表明，EA治疗降低了围产期大鼠的斜坡行为并增加了悬垂行为。该治疗还减少了阳性细胞的数量。CBS的激活剂和抑制剂分别加重和减轻了皮质细胞的缺氧损伤。EA治疗降低了围产期大鼠皮质细胞中CBS的表达水平，并增加了HO-1和HIF-1α的表达水平。与对照组相比，EA治疗组皮质细胞的CO含量显著增加（**p < 0.01）。我们推测，EA治疗通过硫化氢/CBS-CO/HO-1-HIF-1α系统增加皮质CO含量以保护免受缺氧损伤。本研究为EA治疗提供了重要参考，并提示了一种新的脑瘫保护机制。
