Kennedy Brendan F, Malheiro Francisco Gomes, Chin Lixin, Sampson David D
The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
The University of Western Australia, School of Electrical, Electronic and Computer Engineering, Optical+Biomedical Engineering Laboratory, 35 Stirling Highway, Crawley, Western Australia 6009, AustraliabThe University of Western Australia, Centre for Micr.
J Biomed Opt. 2014;19(7):076006. doi: 10.1117/1.JBO.19.7.076006.
We present an acquisition method for optical coherence elastography (OCE) that enables acquisition of three-dimensional elastograms in 5 s, an order of magnitude faster than previously reported. In this method, based on compression elastography, the mechanical load applied to the sample is altered between acquisitions of consecutive optical coherence tomography volume scans (C-scans). The voxel-by-voxel phase difference between the volumes is used to determine the axial displacement and determining the gradient of the axial displacement versus depth gives the local axial strain. We demonstrate sub-100-microstrain sensitivity and high contrast in elastograms, acquired in 5 s, of structured phantoms and freshly excised rat muscle tissue that are comparable in strain sensitivity and dynamic range to our previously reported B-scan-based method. The much higher acquisition speed may expedite the translation of OCE to clinical and in vivo applications.
我们提出了一种用于光学相干弹性成像（OCE）的采集方法，该方法能够在5秒内采集三维弹性图，比之前报道的速度快一个数量级。在这种方法中，基于压缩弹性成像，在连续的光学相干断层扫描体积扫描（C扫描）采集之间改变施加到样本上的机械负载。各体积之间逐体素的相位差用于确定轴向位移，而确定轴向位移相对于深度的梯度可得出局部轴向应变。我们展示了在5秒内采集的结构化体模和新鲜切除的大鼠肌肉组织弹性图中的亚100微应变灵敏度和高对比度，其应变灵敏度和动态范围与我们之前报道的基于B扫描的方法相当。更高的采集速度可能会加快OCE向临床和体内应用的转化。
