Chagnon-Choquet Josiane, Fontaine Julie, Poudrier Johanne, Roger Michel
Laboratoire d'Immunogénétique, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montréal, Quebec, Canada; Département de Microbiologie, Infectiologie et Immunologie de l'Université de Montréal, Montréal, Quebec, Canada.
PLoS One. 2014 Jul 8;9(7):e101949. doi: 10.1371/journal.pone.0101949. eCollection 2014.
Understanding how the immune system facilitates or controls HIV-1 disease progression has important implications for the design of effective interventions. We report that although B-cell dysregulations associated with HIV-1 disease progression are accompanied by an overall decrease in the percentage of total blood B-cells, we observe an increase in relative frequencies of cells presenting characteristics of both transitional immature and first-line marginal zone (MZ) B-cell populations, we designated as precursor MZ-like B-cells. B-cells with similar attributes have been associated with IL-10 expression and "regulatory" potential. As such, the relative frequencies of precursor MZ-like B-cells expressing IL-10 are increased in the blood of viremic HIV-1-infected individuals when compared to HIV-negative subjects. Importantly, in aviremic HIV-1 Elite-Controllers (EC), we found unaltered relative percentages of precursor MZ-like B-cells which presented normal IL-10 expression patterns. Furthermore, EC had increased relative frequencies of blood MZ-like B-cells expressing LT-α. Thus in contrast to viremic HIV-1-infected individuals, EC present MZ-like B-cell populations which IL-10 and LT-α expression profiles may favour homeostasis of immune responses and lymphoid microenvironments.
了解免疫系统如何促进或控制HIV-1疾病进展对于设计有效的干预措施具有重要意义。我们报告称，虽然与HIV-1疾病进展相关的B细胞失调伴随着全血B细胞百分比的总体下降，但我们观察到呈现过渡性未成熟和一线边缘区（MZ）B细胞群体特征的细胞相对频率增加，我们将其指定为前体MZ样B细胞。具有类似特征的B细胞与IL-10表达和“调节”潜力有关。因此，与HIV阴性受试者相比，病毒血症HIV-1感染个体血液中表达IL-10的前体MZ样B细胞相对频率增加。重要的是，在无病毒血症的HIV-1精英控制者（EC）中，我们发现前体MZ样B细胞的相对百分比未改变，其呈现正常的IL-10表达模式。此外，EC中表达LT-α的血液MZ样B细胞相对频率增加。因此，与病毒血症HIV-1感染个体相比，EC呈现出MZ样B细胞群体，其IL-10和LT-α表达谱可能有利于免疫反应和淋巴微环境的稳态。
