Cheng Baoyun, Zhang Yujie, Guo Gongrui, Gao Yongxiang
School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):967-9. doi: 10.1107/S2053230X14011704. Epub 2014 Jun 19.
The Gos1 protein (Golgi SNAP receptor complex member 1) is involved in the SNARE complex, which is the core machinery that drives membrane fusion between cargo-carrying vesicles and their target membranes in the secretory and endocytic pathways in yeast. Truncated versions of the Gos1 protein from Saccharomyces cerevisiae were cloned, expressed, purified and crystallized. The crystal belonged to space group P2₁2₁2₁, with unit-cell parameters a=39.67, b=43.58, c=81.94 Å, α=β=γ=90°. An X-ray diffraction data set was collected at 100 K to 1.63 Å resolution. Matthews coefficient (VM) calculations suggest that one molecule is present in the asymmetric unit, corresponding to a solvent content of ∼55%.
Gos1蛋白（高尔基体可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体复合物成员1）参与SNARE复合物，该复合物是驱动酵母分泌和内吞途径中携带货物的囊泡与其靶膜之间膜融合的核心机制。克隆、表达、纯化并结晶了来自酿酒酵母的Gos1蛋白截短版本。晶体属于空间群P2₁2₁2₁，晶胞参数a = 39.67、b = 43.58、c = 81.94 Å，α = β = γ = 90°。在100 K下收集了分辨率为1.63 Å的X射线衍射数据集。马修斯系数（VM）计算表明，不对称单位中存在一个分子，对应于约55%的溶剂含量。
