与参与酵母核糖体生物合成的Mpp10肽形成复合物的Imp3的纯化、结晶及初步X射线衍射分析
Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis.
作者信息
Zheng Sanduo, Ye Keqiong
机构信息
Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
National Institute of Biological Sciences, Beijing 102206, People's Republic of China.
出版信息
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):918-21. doi: 10.1107/S2053230X14010905. Epub 2014 Jun 18.
Abstract
Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3-Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a=51.6, b=86.9, c=88.7 Å.
摘要
真核生物核糖体的合成需要大量瞬时结合因子。Mpp10、Imp3和Imp4在90S核糖体前体颗粒中形成一个蛋白质复合物，该复合物对18S rRNA进行早期加工。在此，研究人员鉴定出Mpp10的一个短片段可与Imp3结合并增加其溶解度。Imp3-Mpp10复合物经共表达、共纯化和共结晶。初步X射线衍射分析表明，该晶体的衍射分辨率为2.1 Å，属于空间群P2₁2₁2₁，晶胞参数为a = 51.6、b = 86.9、c = 88.7 Å。
相似文献
1
Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide involved in yeast ribosome biogenesis.与参与酵母核糖体生物合成的Mpp10肽形成复合物的Imp3的纯化、结晶及初步X射线衍射分析
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):918-21. doi: 10.1107/S2053230X14010905. Epub 2014 Jun 18.
2
Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome.Mpp10代表了90S前核糖体中多种因子相互作用的一个平台。
PLoS One. 2017 Aug 16;12(8):e0183272. doi: 10.1371/journal.pone.0183272. eCollection 2017.
3
The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60-80S ribonucleoprotein complexes.人类的Imp3和Imp4蛋白与hMpp10形成三元复合物，而hMpp10仅在60 - 80S核糖核蛋白复合物中与U3小核仁RNA相互作用。
Nucleic Acids Res. 2003 Apr 1;31(7):1877-87. doi: 10.1093/nar/gkg300.
4
Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus.Sas10 通过稳定 Mpp10 并将 Mpp10-Imp3-Imp4 复合物递送至核仁来控制核糖体的生物发生。
Nucleic Acids Res. 2019 Apr 8;47(6):2996-3012. doi: 10.1093/nar/gkz105.
5
Mpp10p, a U3 small nucleolar ribonucleoprotein component required for pre-18S rRNA processing in yeast.Mpp10p，一种酵母中前体18S核糖体RNA加工所需的U3小核仁核糖核蛋白组分。
Mol Cell Biol. 1997 Oct;17(10):5803-12. doi: 10.1128/MCB.17.10.5803.
6
Crystallization and preliminary X-ray diffraction analysis of Gos1p, a yeast SNARE protein.酵母SNARE蛋白Gos1p的结晶及初步X射线衍射分析
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):967-9. doi: 10.1107/S2053230X14011704. Epub 2014 Jun 19.
7
Imp3 unfolds stem structures in pre-rRNA and U3 snoRNA to form a duplex essential for small subunit processing.Imp3 展开 pre-rRNA 和 U3 snoRNA 中的茎结构，形成双链体，这对于小亚基加工是必不可少的。
RNA. 2013 Oct;19(10):1372-83. doi: 10.1261/rna.039511.113. Epub 2013 Aug 26.
8
Two-hybrid Mpp10p interaction-defective Imp4 proteins are not interaction defective in vivo but do confer specific pre-rRNA processing defects in Saccharomyces cerevisiae.双杂交Mpp10p相互作用缺陷型Imp4蛋白在体内并非相互作用缺陷，但确实会在酿酒酵母中导致特定的前体rRNA加工缺陷。
Nucleic Acids Res. 2004 Feb 27;32(4):1404-13. doi: 10.1093/nar/gkh318. Print 2004.
9
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.90S 前核糖体的结构：真核核糖体诞生的结构视角。
Cell. 2016 Jul 14;166(2):380-393. doi: 10.1016/j.cell.2016.06.014.
10
Adjacent gene pairing plays a role in the coordinated expression of ribosome biogenesis genes MPP10 and YJR003C in Saccharomyces cerevisiae.相邻基因配对在酿酒酵母核糖体生物合成基因MPP10和YJR003C的协同表达中发挥作用。
Eukaryot Cell. 2011 Jan;10(1):43-53. doi: 10.1128/EC.00257-10. Epub 2010 Nov 29.
引用本文的文献
1
Molecular Response of to Patulin: Transcriptomic-Based Analysis.展青霉素的分子响应：基于转录组学的分析
J Fungi (Basel). 2023 Apr 30;9(5):538. doi: 10.3390/jof9050538.
2
Structural basis for KCTD-mediated rapid desensitization of GABA signalling.KCTD 介导的 GABA 信号快速脱敏的结构基础。
Nature. 2019 Mar;567(7746):127-131. doi: 10.1038/s41586-019-0990-0. Epub 2019 Feb 27.
3
Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus.Sas10 通过稳定 Mpp10 并将 Mpp10-Imp3-Imp4 复合物递送至核仁来控制核糖体的生物发生。
Nucleic Acids Res. 2019 Apr 8;47(6):2996-3012. doi: 10.1093/nar/gkz105.
4
Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.冷冻电镜结构解析揭示了大亚基核糖体前体的半组装中间态
Protein Cell. 2019 Feb;10(2):120-130. doi: 10.1007/s13238-018-0526-7. Epub 2018 Mar 19.
5
The complete structure of the small-subunit processome.小亚基核糖体组装体的完整结构。
Nat Struct Mol Biol. 2017 Nov;24(11):944-953. doi: 10.1038/nsmb.3472. Epub 2017 Sep 25.
6
Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome.Mpp10代表了90S前核糖体中多种因子相互作用的一个平台。
PLoS One. 2017 Aug 16;12(8):e0183272. doi: 10.1371/journal.pone.0183272. eCollection 2017.
7
Molecular architecture of the 90S small subunit pre-ribosome.90S小亚基前核糖体的分子结构
Elife. 2017 Feb 28;6:e22086. doi: 10.7554/eLife.22086.
8
Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network.淋病奈瑟菌铁及铁摄取调节蛋白（Fur）调控网络的特性分析
J Bacteriol. 2016 Jul 28;198(16):2180-91. doi: 10.1128/JB.00166-16. Print 2016 Aug 15.
本文引用的文献
1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Ribosome biogenesis in the yeast Saccharomyces cerevisiae.酵母酿酒酵母中的核糖体生物发生。
Genetics. 2013 Nov;195(3):643-81. doi: 10.1534/genetics.113.153197.
3
Imp3 unfolds stem structures in pre-rRNA and U3 snoRNA to form a duplex essential for small subunit processing.Imp3 展开 pre-rRNA 和 U3 snoRNA 中的茎结构，形成双链体，这对于小亚基加工是必不可少的。
RNA. 2013 Oct;19(10):1372-83. doi: 10.1261/rna.039511.113. Epub 2013 Aug 26.
4
The structure of the eukaryotic ribosome at 3.0 Å resolution.真核生物核糖体的 3.0 Å 分辨率结构。
Science. 2011 Dec 16;334(6062):1524-9. doi: 10.1126/science.1212642. Epub 2011 Nov 17.
5
Transfer-PCR (TPCR): a highway for DNA cloning and protein engineering.转移 PCR（TPCR）：DNA 克隆和蛋白质工程的高速公路。
J Struct Biol. 2011 Aug;175(2):171-7. doi: 10.1016/j.jsb.2011.04.005. Epub 2011 Apr 15.
6
The small subunit processome in ribosome biogenesis—progress and prospects.核糖体生物发生中的小亚基加工体——进展与展望。
Wiley Interdiscip Rev RNA. 2011 Jan-Feb;2(1):1-21. doi: 10.1002/wrna.57.
7
XDS.XDS.（这个词如果没有更多背景信息，很难准确翻译出更有意义的内容，直接保留原文是一种处理方式，或者音译为“克斯达斯”之类，但感觉都不太符合常规翻译场景，你可以补充更多关于这个词的信息以便我更准确翻译 ）
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. doi: 10.1107/S0907444909047337. Epub 2010 Jan 22.
8
RNA chaperones stimulate formation and yield of the U3 snoRNA-Pre-rRNA duplexes needed for eukaryotic ribosome biogenesis.RNA伴侣蛋白刺激真核生物核糖体生物合成所需的U3小核仁RNA-前体rRNA双链体的形成并提高其产量。
J Mol Biol. 2009 Jul 31;390(5):991-1006. doi: 10.1016/j.jmb.2009.05.072. Epub 2009 May 29.
9
Imp3p and Imp4p mediate formation of essential U3-precursor rRNA (pre-rRNA) duplexes, possibly to recruit the small subunit processome to the pre-rRNA.Imp3p和Imp4p介导必需的U3前体核糖体RNA（前体rRNA）双链体的形成，可能是为了将小亚基加工体招募到前体rRNA上。
Proc Natl Acad Sci U S A. 2004 Oct 26;101(43):15301-6. doi: 10.1073/pnas.0406819101. Epub 2004 Oct 15.
10
The human Imp3 and Imp4 proteins form a ternary complex with hMpp10, which only interacts with the U3 snoRNA in 60-80S ribonucleoprotein complexes.人类的Imp3和Imp4蛋白与hMpp10形成三元复合物，而hMpp10仅在60 - 80S核糖核蛋白复合物中与U3小核仁RNA相互作用。
Nucleic Acids Res. 2003 Apr 1;31(7):1877-87. doi: 10.1093/nar/gkg300.
Zotero 插件