用于肿瘤成像的基于MUC1适配体的近红外荧光探针。
MUC1 aptamer-based near-infrared fluorescence probes for tumor imaging.
作者信息
Chen Haiyan, Zhao Juan, Zhang Min, Yang Haibo, Ma Yuxiang, Gu Yueqing
机构信息
Department of Biomedical Engineering, School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009, China.
出版信息
Mol Imaging Biol. 2015 Feb;17(1):38-48. doi: 10.1007/s11307-014-0763-y.
PURPOSE
DNA aptamer (APT) is able to bind to Mucin 1 (MUC1) specifically. The possibility of APT acting as a moiety to construct tumor-targeting probes was investigated.
PROCEDURES
A near-infrared (NIR) fluorescent dye (MPA) and polyethylene glycol (PEG) were conjugated to APT to form APT-MPA and APT-PEG-MPA. The successful synthesis of the two probes was characterized via thin layer chromatography (TLC) and optical spectra. The tumor-targeting efficacy of the probes was evaluated in detail at cell level and animal level, respectively.
RESULTS
The results indicated that MPA and PEG were successfully coupled with APT. APT-based probes were mediated by Mucin 1 into tumor cells, and PEG-modified probe exhibited higher cell affinity.
CONCLUSIONS
The aptamer-based NIR fluorescent probes are promising candidates for tumor imaging and diagnosis.
目的
DNA适配体（APT）能够特异性结合黏蛋白1（MUC1）。研究了APT作为构建肿瘤靶向探针的一部分的可能性。
程序
将近红外（NIR）荧光染料（MPA）和聚乙二醇（PEG）与APT偶联，形成APT-MPA和APT-PEG-MPA。通过薄层色谱（TLC）和光谱对两种探针的成功合成进行了表征。分别在细胞水平和动物水平详细评估了探针的肿瘤靶向效果。
结果
结果表明MPA和PEG成功地与APT偶联。基于APT的探针由黏蛋白1介导进入肿瘤细胞，并且PEG修饰的探针表现出更高的细胞亲和力。
结论
基于适配体的近红外荧光探针是肿瘤成像和诊断的有前途的候选者。
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