Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
Protein Cell. 2012 Oct;3(10):739-54. doi: 10.1007/s13238-012-2072-z. Epub 2012 Sep 15.
Molecular imaging has greatly advanced basic biology and translational medicine through visualization and quantification of single/multiple molecular events temporally and spatially in a cellular context and in living organisms. Aptamers, short single-stranded nucleic acids selected in vitro to bind a broad range of target molecules avidly and specifically, are ideal molecular recognition elements for probe development in molecular imaging. This review summarizes the current state of aptamer-based biosensor development (probe design and imaging modalities) and their application in imaging small molecules, nucleic acids and proteins mostly in a cellular context with some animal studies. The article is concluded with a brief discussion on the perspective of aptamer-based molecular imaging.
分子成像是通过在细胞环境和活体生物中对单个/多个分子事件进行时空可视化和定量，从而极大地推动了基础生物学和转化医学的发展。适体是在体外筛选出的短单链核酸，能够强烈而特异性地结合广泛的靶分子，是分子成像中探针开发的理想分子识别元件。本综述总结了基于适体的生物传感器的发展现状（探针设计和成像方式）及其在小分子、核酸和蛋白质成像中的应用，主要是在细胞环境中，也有一些动物研究。文章最后简要讨论了基于适体的分子成像的前景。
