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猫杯状病毒能够耐受因改变翻译重新起始频率或使用单独的VP2编码mRNA而导致的次要衣壳蛋白表达水平的显著变化。

Feline calicivirus can tolerate gross changes of its minor capsid protein expression levels induced by changing translation reinitiation frequency or use of a separate VP2-coding mRNA.

作者信息

Haβ Maria, Luttermann Christine, Meyers Gregor

机构信息

Institut für Immunologie, Friedrich-Loeffler-Institut, Tübingen, Germany.

出版信息

PLoS One. 2014 Jul 9;9(7):e102254. doi: 10.1371/journal.pone.0102254. eCollection 2014.

DOI:10.1371/journal.pone.0102254
PMID:25007260
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4090194/
Abstract

Caliciviruses use reinitiation of translation governed by a 'termination upstream ribosomal binding site' (TURBS) for expression of their minor capsid protein VP2. Mutation analysis allowed to identify sequences surrounding the translational start/stop site of the feline calicivirus (FCV) that fine tune reinitiation frequency. A selection of these changes was introduced into the infectious FCV cDNA clone to check the influence of altered VP2 levels on virus replication. In addition, full length constructs were established that displayed a conformation, in which VP2 expression occurred under control of a duplicated subgenomic promoter. Viable viruses recovered from such constructs revealed a rather broad range of VP2 expression levels but comparable growth kinetics showing that caliciviruses can tolerate gross changes of the VP2 expression level.

摘要

杯状病毒利用由“上游终止核糖体结合位点”（TURBS）控制的翻译重新起始来表达其次要衣壳蛋白VP2。突变分析有助于鉴定猫杯状病毒（FCV）翻译起始/终止位点周围的序列，这些序列可微调重新起始频率。将这些变化中的一部分引入感染性FCV cDNA克隆中，以检查VP2水平改变对病毒复制的影响。此外，还构建了全长构建体，其呈现出一种构象，其中VP2的表达受重复的亚基因组启动子控制。从这些构建体中回收的活病毒显示出相当广泛的VP2表达水平范围，但生长动力学相当，这表明杯状病毒能够耐受VP2表达水平的大幅变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/2799ace73f22/pone.0102254.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/559c1e41d408/pone.0102254.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/3d99d0713d80/pone.0102254.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/384fda8716bc/pone.0102254.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/1fc327ac0abc/pone.0102254.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/81d876b028ee/pone.0102254.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/2799ace73f22/pone.0102254.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/559c1e41d408/pone.0102254.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/3d99d0713d80/pone.0102254.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/384fda8716bc/pone.0102254.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/1fc327ac0abc/pone.0102254.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/81d876b028ee/pone.0102254.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ab0/4090194/2799ace73f22/pone.0102254.g007.jpg

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Please do not recycle! Translation reinitiation in microbes and higher eukaryotes.请勿回收！微生物和高等真核生物中的翻译重新起始。

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