Sun Xiulan, He Xingxing, Xue Kathy siyu, Li Yun, Xu Dan, Qian He
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, China.
Food Chem Toxicol. 2014 Oct;72:76-82. doi: 10.1016/j.fct.2014.06.021. Epub 2014 Jul 5.
Zearalenone (ZEN) contamination of corn and cereal products is a serious health hazard throughout the world and its elimination by microbial methods is now being widely examined. In this study, an Aspergillus niger strain, FS10, isolated from Chinese fermented soybean, was shown to reduce levels of ZEN in corn steep liquor (CSL). Spores, mycelium and culture filtrate of the strain FS10 were tested for their ability to remove ZEN. The results indicated that strain FS10 could remove 89.56% of ZEN from potato dextrose broth (PDB) medium. Mycelium and culture filtrate decreased the ZEN content by 43.10% and 68.16%, respectively. The contaminated corn steep liquor initially contained ZEN 29 μg/ml, 60.01% of which could be removed by strain FS10. To demonstrate the loss of toxicity in vivo, the culture filtrate incubated with the contaminated corn steep liquor for 48 h was administered to rats. The results indicated that the contaminated corn steep liquor severely damaged liver and kidney tissue. Rats administered with contaminated corn steep liquor treated with the strain FS10 culture filtrate showed significantly less severe liver and kidney damage, and organ index values were comparable to the non-ZEN-exposed control (p<0.05). Our study suggests an effective approach to reduce the hazards of ZEN in corn steep liquor.
玉米和谷物产品中的玉米赤霉烯酮（ZEN）污染在全球范围内都是严重的健康危害，目前人们正在广泛研究通过微生物方法消除这种污染。在本研究中，从中国发酵大豆中分离出的一株黑曲霉FS10被证明可以降低玉米浆（CSL）中ZEN的含量。对FS10菌株的孢子、菌丝体和培养滤液去除ZEN的能力进行了测试。结果表明，FS10菌株可以从马铃薯葡萄糖肉汤（PDB）培养基中去除89.56%的ZEN。菌丝体和培养滤液分别使ZEN含量降低了43.10%和68.16%。受污染的玉米浆最初含有29 μg/ml的ZEN，其中60.01%可被FS10菌株去除。为了证明体内毒性的丧失，将与受污染玉米浆孵育48小时的培养滤液给大鼠服用。结果表明，受污染的玉米浆严重损害了肝脏和肾脏组织。用FS10菌株培养滤液处理过的受污染玉米浆给药的大鼠，其肝脏和肾脏损伤明显减轻，器官指数值与未接触ZEN的对照组相当（p<0.05）。我们的研究提出了一种有效降低玉米浆中ZEN危害的方法。
