Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Toxins (Basel). 2018 Oct 31;10(11):449. doi: 10.3390/toxins10110449.
Zearalenone (ZEN) is an estrogen-like mycotoxin produced by that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice ( = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum ( < 0.05; < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group ( < 0.05; < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice.
玉米赤霉烯酮（ZEN）是一种雌激素样真菌毒素，由某些真菌产生，严重危及动物和人类健康。本研究旨在评估 A2 对 ZEN 诱导的昆明小鼠生化和病理变化的保护作用。将 40 只昆明小鼠（体重 25 ± 2 g）分为 4 个处理组：对照组（基础饲料）；ZEN 组（基础饲料加 ZEN 剂量 60 mg/kg）；A2 发酵饲料组（150 g 饲料混合 150 mL 无菌蒸馏水，接种 5 mL 磷酸盐缓冲盐（PBS）悬浮 A2 菌株）；A2 发酵 ZEN 污染饲料组。（A2 株组 150 mL 纯细菌蒸馏水系统混合 150 g ZEN 污染饲料。）结果表明，A2 株在 5 天内可完全降解 ZEN 污染饲料。（发酵液中 ZEN 浓度为 60 μg/mL。）小鼠连续喂养 28 天后，与对照组相比，ZEN 组血清中天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性升高，谷胱甘肽过氧化物酶（GSH-PX）和总超氧化物歧化酶（T-SOD）活性降低，肌酐（CRE）、血尿素氮（BUN）、尿酸（UA）和丙二醛（MDA）含量增加（ < 0.05； < 0.01）。然而，与 ZEN 组相比，A2 发酵饲料组和 A2 发酵 ZEN 污染饲料组的这些生化水平均有所逆转（ < 0.05； < 0.01）。此外，组织病理学分析仅显示 ZEN 组小鼠肝脏有病理变化。结果表明，A2 作为添加剂可以有效去除饲料中的 ZEN 污染，并保护小鼠免受 ZEN 的毒性损害。综上所述，A2 作为微生物饲料添加剂具有很大的应用潜力，可在生产实践中解毒 ZEN 的毒性。
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