Nicolella Heloiza Diniz, de Oliveira Pollyanna Francielli, Munari Carla Carolina, Costa Gizela Faleiros Dias, Moreira Monique Rodrigues, Veneziani Rodrigo Cassio Sola, Tavares Denise Crispim
Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil.
Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 - Parque Universitário, 14404-600 Franca, São Paulo, Brazil.
Food Chem Toxicol. 2014 Oct;72:8-12. doi: 10.1016/j.fct.2014.06.025. Epub 2014 Jul 5.
Salvia officinalis (sage) is a perennial woody subshrub native to the Mediterranean region that is commonly used as a condiment and as an anti-inflammatory, antioxidant and antimicrobial agent due to its biological activities. Manool is the most abundant micro-metabolite found in Salvia officinalis essential oils and extracts. We therefore decided to evaluate the cytotoxic, genotoxic and antigenotoxic potential of manool in Chinese hamster lung fibroblasts (V79) and human hepatoma cells (HepG2). Cytotoxicity was assessed by the colony-forming assay in V79 cells and toxic effects were observed at concentrations of up to 8.0 μg/mL. The micronucleus test was used to evaluate the genotoxicity and antigenotoxicity of manool in V79 and HepG2 cells at concentrations of 0.5-6.0 μg/mL and 0.5-8.0 μg/mL, respectively. For evaluation of antigenotoxicity, the concentrations of manool were combined with methyl methanesulfonate (MMS, 44 μg/mL). The results showed a significant increase in the frequency of micronuclei in cultures of both cell lines treated with the highest concentration tested, demonstrating a genotoxic effect. On the other hand, manool exhibited a protective effect against chromosome damage induced by MMS in HepG2 cells, but not in V79 cells. These data suggest that some manool metabolite may be responsible for the antigenotoxic effect observed in HepG2 cells.
鼠尾草是一种多年生木本亚灌木，原产于地中海地区，因其生物活性，常用于调味品，也用作抗炎、抗氧化和抗菌剂。异海松醇是鼠尾草精油和提取物中含量最丰富的微量代谢物。因此，我们决定评估异海松醇对中国仓鼠肺成纤维细胞（V79）和人肝癌细胞（HepG2）的细胞毒性、遗传毒性和抗遗传毒性潜力。通过V79细胞的集落形成试验评估细胞毒性，在高达8.0 μg/mL的浓度下观察到毒性作用。微核试验分别用于评估浓度为0.5-6.0 μg/mL的异海松醇对V79细胞和浓度为0.5-8.0 μg/mL的异海松醇对HepG2细胞的遗传毒性和抗遗传毒性。为了评估抗遗传毒性，将异海松醇的浓度与甲基磺酸甲酯（MMS，44 μg/mL）联合使用。结果显示，在两种细胞系中，用最高测试浓度处理的培养物中微核频率显著增加，表明具有遗传毒性作用。另一方面，异海松醇对HepG2细胞中由MMS诱导的染色体损伤具有保护作用，但对V79细胞没有保护作用。这些数据表明，某些异海松醇代谢物可能是在HepG2细胞中观察到的抗遗传毒性作用的原因。
