Wang Qiuling, Xiao Peigen, Luo Kun, Song Jingyuan, Wei Shengli, Jian Zaiyou, Hou Junling, Peng Yong, Wang Wenquan
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College.
Biol Pharm Bull. 2014;37(9):1516-24. doi: 10.1248/bpb.b14-00335. Epub 2014 Jul 9.
One hundred and forty-four samples of Chishao and Baishao, which represented six species of Paeonia L. were evaluated for their genetic variation, genetic differentiation and phylogenetic relationship, based on the nuclear ribosomal DNA internal transcribed spacer (ITS) region. Samples from representative of the population were then used to do a cultivation comparison experiment, and then to identify the contents of the active ingredients. The results showed there were differences in the haplotype distribution and frequency between populations of Chishao and Baishao. An analysis of molecular variance (AMOVA) indicated statistically significant (p<0.001) genetic differentiation between the populations of wild and cultivated Paeonia lactiflora PALL. The albiflorin content between Chishao and Baishao was also significantly different (p<0.05). All the results clearly illustrate that currently cultivated P. lactiflora cannot be used as a substitute for Chishao.
基于核糖体DNA内转录间隔区（ITS）区域，对代表芍药属6个物种的144份赤芍和白芍样本进行了遗传变异、遗传分化和系统发育关系评估。然后使用来自代表性种群的样本进行栽培比较实验，进而鉴定活性成分的含量。结果表明，赤芍和白芍种群之间的单倍型分布和频率存在差异。分子方差分析（AMOVA）表明，野生和栽培芍药种群之间存在统计学上显著的（p<0.001）遗传分化。赤芍和白芍之间的芍药苷含量也存在显著差异（p<0.05）。所有结果清楚地表明，目前栽培的芍药不能用作赤芍的替代品。
