Xu Shiyi, Liu Weili, Liu Xiubo, Qin Chen, He Lianqing, Wang Panpan, Kong Lingyang, Chen Xi, Liu Zhiyang, Ma Wei
School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China.
Experimental Training Center, Heilongjiang University of Chinese Medicine, Harbin, China.
Front Chem. 2023 Mar 10;11:1158727. doi: 10.3389/fchem.2023.1158727. eCollection 2023.
Intersubgeneric hybrids of ( pall., ) cover a huge variety of systems in the genus . In recent years, many studies have confirmed that the intersubgeneric hybrids of are rich in paeoniflorin and other medicinal ingredients, however, it has always proved difficult to clarify the medicinal value of the hybrids and whether they can be used for medicinal purposes. In this study, the consistency of the plant population was evaluated through DUS evaluation, in order to clarify whether the selected research materials had stability and consistency within the population and specificity between populations. The differences between the paeoniflorin contents in the roots of the nine intersubgeneric hybrids of the varieties and two medicinal varieties were critically compared. The differences in the chemical components of the roots of nine intersubgeneric hybrids of and reference medicine substances of and subsp. (Lynch) D. Y. Hong and K. Y. Pan (., .) were explored stoichiometric and chemical fingerprint high performance liquid chromatography analyses. The results showed that there were significant differences in the chemical compositions between the intersubgeneric hybrids of . and the medicinal reference materials, and the contents of paeoniflorin were elevated such that the hybrids could be used as the raw material for extraction of paeoniflorin, thus providing an opportunity to explore the medicinal value of the hybrids. This study explored the key differential components among the varieties and provides a reference and basis for the study of the medicinal value and the identification of the intersubgeneric hybrids of the varieties.
芍药属（Paeonia L.）种间杂种涵盖了该属内极为多样的系统。近年来，诸多研究证实芍药属种间杂种富含芍药苷及其他药用成分，然而，一直以来都难以阐明这些杂种的药用价值以及它们是否可用于药用目的。在本研究中，通过DUS评价对植物群体的一致性进行了评估，以明确所选取的研究材料在群体内是否具有稳定性和一致性以及群体间是否具有特异性。对芍药属9个种间杂种品种与2个药用品种根中芍药苷含量的差异进行了严格比较。通过化学计量学和化学指纹图谱高效液相色谱分析，探究了芍药属9个种间杂种根的化学成分与芍药属（Paeonia lactiflora Pall.）及川赤芍（Paeonia veitchii Lynch）的参考药用物质（P. lactiflora Pall.，P. veitchii Lynch）之间的差异。结果表明，芍药属种间杂种与药用参考材料之间的化学成分存在显著差异，且芍药苷含量有所提高，使得这些杂种可作为提取芍药苷的原料，从而为探索杂种的药用价值提供了契机。本研究探究了各品种间的关键差异成分，为芍药属品种的药用价值研究及种间杂种鉴定提供了参考和依据。
