Lu Xiao-Ming, Lu Peng-Zhen
Institute for Eco-Environmental Sciences, Wenzhou Vocational College of Science and Technology, Wenzhou, 325006, China,
Microb Ecol. 2014 Nov;68(4):773-84. doi: 10.1007/s00248-014-0456-8. Epub 2014 Jul 10.
The pyrosequencing technique was used to evaluate bacterial community structures in sediment and surface water samples taken from Nanxi River receiving effluents from a paper mill and a farmhouse hotel, respectively. For each sample, 4,610 effective bacterial sequences were selected and used to do the analysis of diversity and abundance, respectively. Bacterial phylotype richness in the sediment sample without effluent input was higher than the other samples, and the surface water sample with addition of effluent from the paper mill contained the least richness. Effluents from both the paper mill and farmhouse hotel have a potential to reduce the bacterial diversity and abundance in the sediment and surface water, especially it is more significant in the sediment. The effect of the paper mill effluent on the sediment and surface water bacterial communities was more serious than that of the farmhouse hotel effluent. Characterization of microbial community structures in the sediment and surface water from two tributaries of the downstream river indicated that various effluents from the paper mill and farmhouse hotel have the similar potential to decrease the natural variability in riverine microbial ecosystems.
焦磷酸测序技术用于评估楠溪江沉积物和地表水样本中的细菌群落结构，这些样本分别取自接纳造纸厂和农家乐酒店废水的区域。对于每个样本，分别选取4610条有效的细菌序列用于多样性和丰度分析。无废水输入的沉积物样本中的细菌系统发育型丰富度高于其他样本，而添加造纸厂废水的地表水样本中丰富度最低。造纸厂和农家乐酒店的废水均有可能降低沉积物和地表水的细菌多样性和丰度，尤其是在沉积物中更为显著。造纸厂废水对沉积物和地表水细菌群落的影响比农家乐酒店废水更为严重。对下游河流两条支流的沉积物和地表水微生物群落结构特征分析表明，造纸厂和农家乐酒店的各种废水具有相似的降低河流微生物生态系统自然变异性的潜力。
