[西安男男性行为人群中与1型人类免疫缺陷病毒感染相关的分子流行病学研究]

[Molecular epidemiology related to human immunodeficiency virus type 1 infection in men having sex with men in Xi'an].

作者信息

Zhang Hailan, Yan Yongping, Li Hengxin, Li Duan, Wei Xiaoli, Zheng Haichao, Zhao Xin

机构信息

The Fourth Military Medical University, Xi'an 710032, China; Xi'an Center for Disease Control and Prevention.

The Fourth Military Medical University, Xi'an 710032, China. Email:

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2014 Apr;35(4):421-4.

PMID:25009033

Abstract

OBJECTIVE

To investigate the subtype distribution of HIV-1 infection among men having sex with men (MSM) in Xi'an city.

METHODS

5 ml anti-coagulating blood samples were collected from MSM who had been reported during 2010-2012 in Xi'an. Both gag and env genes were amplified by nested RT-PCR from the extracted RNA and then sequenced. The acquired sequences were compared with international subtype references, and then the genetic distances were calculated and phylogenetic trees were constructed by Mega 5.2. Epidemiological information including sexual behavior characteristics, history of drug use, blood donation, etc. were gathered.

RESULTS

168 samples were successfully amplified and sequenced. Results from Phylogenic analysis showed that the gag and env sequences of 165 samples shared the same subtypes, of which 79 (47.0%) were CRF01_AE, 74 (44.0%) were CRF07_BC and 12 (7.1%) belonged to subtype B. There were 3 samples with gag and env sequences classified into different subtypes, of which 2(1.2%) were CRF01_AE/A1, and the other 1 was CRF07_BC/CRF01_AE.

CONCLUSION

At least three HIV-1 subtypes including CRF01_AE, CRF07_BC were identified among MSM population in Xi'an city.

摘要

目的

调查西安市男男性行为者（MSM）中HIV-1感染的亚型分布情况。

方法

收集2010 - 2012年西安市报告的MSM的5ml抗凝血液样本。从提取的RNA中通过巢式RT-PCR扩增gag和env基因，然后进行测序。将获得的序列与国际亚型参考序列进行比较，计算遗传距离，并使用Mega 5.2构建系统发育树。收集包括性行为特征、吸毒史、献血史等流行病学信息。

结果

成功扩增并测序168个样本。系统发育分析结果显示，165个样本的gag和env序列属于同一亚型，其中79个（47.0%）为CRF01_AE，74个（44.0%）为CRF07_BC，12个（7.1%）属于B亚型。有3个样本的gag和env序列被分类为不同亚型，其中2个（1.2%）为CRF01_AE/A1，另1个为CRF07_BC/CRF01_AE。