LaFave Matthew C, Varshney Gaurav K, Vemulapalli Meghana, Mullikin James C, Burgess Shawn M
Developmental Genomics Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892.
National Institutes of Health Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20814.
Genetics. 2014 Sep;198(1):167-70. doi: 10.1534/genetics.114.166769. Epub 2014 Jul 9.
Substantial intrastrain variation at the nucleotide level complicates molecular and genetic studies in zebrafish, such as the use of CRISPRs or morpholinos to inactivate genes. In the absence of robust inbred zebrafish lines, we generated NHGRI-1, a healthy and fecund strain derived from founder parents we sequenced to a depth of ∼50×. Within this strain, we have identified the majority of the genome that matches the reference sequence and documented most of the variants. This strain has utility for many reasons, but in particular it will be useful for any researcher who needs to know the exact sequence (with all variants) of a particular genomic region or who wants to be able to robustly map sequences back to a genome with all possible variants defined.
斑马鱼在核苷酸水平上存在显著的株内变异，这使得分子和遗传研究变得复杂，例如使用CRISPR或吗啉代寡核苷酸使基因失活。由于缺乏稳定的近交斑马鱼品系，我们培育了NHGRI-1，这是一个健康且繁殖力强的品系，其奠基亲本的测序深度约为50倍。在这个品系中，我们已经确定了与参考序列匹配的大部分基因组，并记录了大多数变异。这个品系有多种用途，特别是对于任何需要知道特定基因组区域的确切序列（包括所有变异）的研究人员，或者想要能够将序列可靠地映射到定义了所有可能变异的基因组的研究人员来说，它将非常有用。
