Sadamitsu Kenichiro, Kashima Makoto, Wada Seiji, Ishioka Akiko, Nakayama Satomi, Nakayama Ryoko, Okamoto Hitoshi, Hirata Hiromi
Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, 252-5258, Japan.
Faculty of Science, Toho University, Funabashi, 274-8510, Japan.
Sci Rep. 2025 Apr 25;15(1):14512. doi: 10.1038/s41598-025-98674-w.
Zebrafish have emerged as an alternative vertebrate model for both basic and applied science. While two zebrafish lines, AB and TU, have been extensively used, recent studies suggest that complex behaviors and susceptibility to adult phenotypes vary among lines. Given the increasing demand across diverse research fields, establishing a phylogenetically distinct wild-type zebrafish line without deleterious genetic variants would greatly benefit the research community. In this study, we documented the establishment of the RIKEN Wild-type (RW) line and conducted comparative genome analyses to investigate the genetic characteristics of various wild-type zebrafish lines such as AB, TU, TL, WIK, SAT, NHGRI-1, PET, *AB, IND, M-AB and IM with a particular focus on the genetic characterization of the RW line. We identified numerous genetic variants in each line that may affect coding proteins, some of which are unique to each line, conferring specific genetic traits. Notably, the RW line was found to carry such genetic variants in 13 genes. Furthermore, our phylogenetic analysis revealed that the RW line is genetically distinct from other commonly used lines. Collectively, the RW line is a robust zebrafish line with excellent breeding characteristics, making it valuable for studies exploring genetic diversity and line-specific traits within the species.
斑马鱼已成为基础科学和应用科学领域的一种替代脊椎动物模型。虽然AB和TU这两个斑马鱼品系已被广泛使用，但最近的研究表明，不同品系之间复杂行为和成年表型易感性存在差异。鉴于不同研究领域的需求不断增加，建立一个没有有害遗传变异的系统发育上不同的野生型斑马鱼品系将极大地造福于研究界。在本研究中，我们记录了理研野生型（RW）品系的建立，并进行了比较基因组分析，以研究各种野生型斑马鱼品系（如AB、TU、TL、WIK、SAT、NHGRI-1、PET、*AB、IND、M-AB和IM）的遗传特征，特别关注RW品系的遗传特征。我们在每个品系中鉴定出许多可能影响编码蛋白的遗传变异，其中一些是每个品系特有的，赋予了特定的遗传特性。值得注意的是，发现RW品系在13个基因中携带此类遗传变异。此外，我们的系统发育分析表明，RW品系在遗传上与其他常用品系不同。总体而言，RW品系是一个具有优良繁殖特性的强健斑马鱼品系，对于探索该物种内遗传多样性和品系特异性性状的研究具有重要价值。
