Department of Cardiology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu Province 210006, China.
J Geriatr Cardiol. 2014 Jun;11(2):141-50. doi: 10.3969/j.issn.1671-5411.2014.02.003.
To clarify the association between rs1050450 polymorphism in Glutathione peroxidase-1 (GPx-1) and the risk of cardiovascular diseases (CVD) by performing a meta-analysis of published studies. There is growing evidence from different study types for an association of the GPx-1 polymorphism and cardiovascular outcomes, but observational studies have so far shown inconsistent results.
Relevant publications were searched through PubMed, Embase database databases and the Cochrane Library. We used odds ratios (ORs) with 95% confidence intervals (CIs) to assess the strength of association under the best genetic model. Both Q statistic and the I (2) were used to check heterogeneity. Meta-regression analysis was performed to explore heterogeneity source. Sensitivity analysis, cumulative meta-analysis analysis and publication bias were used to test the reliability of the results.
Data were available from two cohort studies and 8 case-control studies involving 1,430 cases and 3,767 controls. The pooled ORs for overall CVD risk was 1.36 with 95% CI: 1.08-1.70 under a co-dominant model, and that for East Asian subgroup was 1.84 (95% CI: 1.39-2.43). Substantial heterogeneity for ORs were detected among all the included studies, mainly caused by ethnic differences between East Asian and non-East Asian populations. Although Egger's regression test suggested no statistical significant publication bias, Begg's funnel plot exhibited obvious asymmetry. The statistical significance disappeared after adjusting for potential publication bias in the overall studies. However, no substantial publication bias was found in the East Asian subgroup.
GPx-1 gene Pro198Leu and Pro197Leu polymorphisms considerably increased the risk of CVD in the East Asian population. Large-scale investigations are needed to confirm the results in different ethnicities.
通过对已发表研究进行荟萃分析，阐明谷胱甘肽过氧化物酶 1（GPx-1）基因 rs1050450 多态性与心血管疾病（CVD）风险之间的关联。不同研究类型的越来越多的证据表明 GPx-1 多态性与心血管结局之间存在关联，但观察性研究迄今为止显示出不一致的结果。
通过 PubMed、Embase 数据库和 Cochrane 图书馆检索相关文献。我们使用最佳遗传模型下的优势比（OR）和 95%置信区间（CI）来评估关联的强度。使用 Q 统计量和 I（2）来检查异质性。进行 meta 回归分析以探索异质性来源。敏感性分析、累积 meta 分析和发表偏倚用于测试结果的可靠性。
有两项队列研究和 8 项病例对照研究的数据可用，涉及 1430 例病例和 3767 例对照。在显性模型下，总体 CVD 风险的合并 OR 为 1.36，95%CI：1.08-1.70，东亚亚组的 OR 为 1.84（95%CI：1.39-2.43）。所有纳入研究的 OR 均存在显著异质性，主要是由于东亚和非东亚人群之间的种族差异所致。虽然 Egger 回归检验表明不存在统计学上显著的发表偏倚，但 Begg 漏斗图显示明显的不对称性。在对总体研究进行潜在发表偏倚调整后，统计显著性消失。然而，东亚亚组未发现明显的发表偏倚。
GPx-1 基因 Pro198Leu 和 Pro197Leu 多态性显著增加了东亚人群 CVD 的风险。需要进行大规模调查以确认不同种族中的结果。
