蒸制发酵党参的神经保护作用。
Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata.
机构信息
Department of Medical Biomaterials Engineering, College of Biomedical Science, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 200-701.
出版信息
Biomol Ther (Seoul). 2014 May;22(3):246-53. doi: 10.4062/biomolther.2014.019.
Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuro-protective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, Ca(2+) and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.
党参已被用作治疗几种肺部炎症性疾病的草药，如哮喘、扁桃体炎和咽炎。此前，我们已经证明了蒸制和发酵党参（SFC）在体外和体内的神经保护作用。在本研究中，SFC 处理 HT22 细胞可减少谷氨酸诱导的细胞死亡，表明 SFC 可保护 HT22 细胞免受谷氨酸诱导的细胞毒性。基于这些，我们试图通过测量 HT22 细胞中的氧化应激参数和 Bax 和 caspase-3 的表达来阐明 SFC 的神经保护作用的机制。SFC 降低了 ROS、Ca（2+）和 NO 的含量。此外，SFC 恢复了谷胱甘肽和谷胱甘肽还原酶的含量，并抑制了 Bax 和 caspase-3 在 HT22 细胞中的活性。这些结果表明，蒸制和发酵党参（SFC）提取物通过抗氧化作用和抑制 Bax 和 caspase-3 的表达来保护 HT22 细胞。
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