Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou 510650, China.
University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, China.
Molecules. 2017 Oct 9;22(10):1655. doi: 10.3390/molecules22101655.
Plant growth retardant (PGR) refers to organics that can inhibit the cell division of plant stem tip sub-apical meristem cells or primordial meristem cell. They are widely used in the cultivation of rhizomatous functional plants; such as that is a famous Chinese traditional herb. However, it is still unclear whether PGR affects the medicinal quality of . In the present study, amino acid analyses, targeted and non-targeted analyses by ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-TOF-MS) and gas chromatography-MS were used to analyze and compare the composition of untreated and treated with PGR. The contents of two key bioactive compounds, lobetyolin and atractylenolide III, were not affected by PGR treatment. The amounts of polysaccharides and some internal volatiles were significantly decreased by PGR treatment; while the free amino acids content was generally increased. Fifteen metabolites whose abundance were affected by PGR treatment were identified by UPLC-TOF-MS. Five of the up-regulated compounds have been reported to show immune activity, which might contribute to the healing efficacy ("buqi") of . The results of this study showed that treatment of with PGR during cultivation has economic benefits and affected some main bioactive compounds in .
植物生长延缓剂（PGR）是指能够抑制植物茎尖亚顶端分生组织细胞或原始分生组织细胞分裂的有机物。它们被广泛应用于根茎状功能性植物的栽培中；例如，它是一种著名的中国传统草药。然而，PGR 是否会影响 的药用质量仍不清楚。在本研究中，采用氨基酸分析、超高效液相色谱-飞行时间质谱（UPLC-TOF-MS）和气相色谱-质谱联用的靶向和非靶向分析方法，分析和比较了未经 PGR 处理的 和用 PGR 处理的 的成分。两种关键生物活性化合物，lobetyolin 和 atractylenolide III 的含量不受 PGR 处理的影响。PGR 处理显著降低了多糖和一些内部挥发物的含量；而游离氨基酸的含量普遍增加。通过 UPLC-TOF-MS 鉴定出 15 种受 PGR 处理影响的代谢产物。其中上调的五种化合物具有免疫活性，这可能有助于 的愈合功效（“buqi”）。本研究结果表明，在栽培过程中用 PGR 处理 具有经济效益，并影响 的一些主要生物活性化合物。
