Maejima Hideki, Nagashio Ryo, Yanagita Kengo, Hamada Yuko, Amoh Yasuyuki, Sato Yuichi, Katsuoka Kensei
Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Japan.
Department of Applied Tumor Pathology, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan; Department of Molecular Diagnostics, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
PLoS One. 2014 Jul 10;9(7):e101773. doi: 10.1371/journal.pone.0101773. eCollection 2014.
To identify diagnostic markers for psoriasis vulgaris and psoriatic arthritis, autoantibodies in sera from psoriasis vulgaris and psoriatic arthritis patients were screened by two-dimensional immunoblotting (2D-IB). Based on 2D-IB and MADLI TOF/TOF-MS analyses, eleven proteins each in psoriasis vulgaris and psoriatic arthritis were identified as autoantigens. Furthermore, serum levels of moesin, keratin 17 (K17), annexin A1 (ANXA1), and stress-induced phophoprotein-1 (STIP1), which were detected as autoantigens, were studied by dot blot analysis with psoriasis patients and healthy controls. The levels of moesin and STIP1 were significantly higher in sera from patients with psoriasis vulgaris than in the controls (moesin: P<0.05, STIP1: P<0.005). The area under the curve (AUC) for moesin and STIP1 between patients with psoraisis vulgaris and controls was 0.747 and 0.792, respectively. STIP1 and K17 levels were significantly higher in sera from patients with psoriatic arthritis than in those with psoriasis vulgaris (P<0.05 each). The AUC for STIP1 and K17 between patients with psoriatic arthritis and psoriasis vulgaris was 0.69 and 0.72, respectively. The STIP1 or moesin, CK17 serum level was not correlated with disease activity of psoriasis patients. These data suggest that STIP1 and moesin may be novel and differential sero-diagnostic markers for psoriasis vulgaris and psoriatic arthritis.
为了鉴定寻常型银屑病和银屑病关节炎的诊断标志物，通过二维免疫印迹法（2D-IB）筛选了寻常型银屑病和银屑病关节炎患者血清中的自身抗体。基于2D-IB和基质辅助激光解吸电离飞行时间串联质谱（MALDI TOF/TOF-MS）分析，分别在寻常型银屑病和银屑病关节炎中鉴定出11种蛋白质作为自身抗原。此外，通过斑点印迹分析研究了作为自身抗原检测到的埃兹蛋白、角蛋白17（K17）、膜联蛋白A1（ANXA1）和应激诱导磷蛋白-1（STIP1）在银屑病患者和健康对照血清中的水平。寻常型银屑病患者血清中埃兹蛋白和STIP1的水平显著高于对照组（埃兹蛋白：P<0.05，STIP1：P<0.005）。寻常型银屑病患者和对照组之间埃兹蛋白和STIP1的曲线下面积（AUC）分别为0.747和0.792。银屑病关节炎患者血清中STIP1和K17的水平显著高于寻常型银屑病患者（均为P<0.05）。银屑病关节炎患者和寻常型银屑病患者之间STIP1和K17的AUC分别为0.69和0.72。STIP1或埃兹蛋白、CK17血清水平与银屑病患者的疾病活动无关。这些数据表明，STIP1和埃兹蛋白可能是寻常型银屑病和银屑病关节炎新的差异血清诊断标志物。
