Ajioka Itsuki, Jinnou Hideo, Okada Kei, Sawada Masato, Saitoh Shinji, Sawamoto Kazunobu
1 Center for Brain Integration Research, Tokyo Medical and Dental University , Tokyo, Japan .
Tissue Eng Part A. 2015 Jan;21(1-2):193-201. doi: 10.1089/ten.TEA.2014.0080. Epub 2014 Aug 20.
After brain injury, neuroblasts generated from endogenous neural stem cells migrate toward the injured site using blood vessels as a scaffold, raising the possibility of reconstructing blood vessel network scaffolds as a strategy for promoting endogenous neuronal regeneration. In this study, we designed biomaterials based on the components and morphology of blood vessel scaffolds, and examined their ability to guide the migration of neuroblasts into a brain lesion site in mice. Transplanted porous sponge containing components of the basement membrane (BM) matrix enhanced neuroblast migration into the lesion, and detailed morphological examination suggested that the infiltrating cells used the BM sponge as a migration scaffold. Laminin (LN)-rich porous sponge also enhanced the migration of neuroblasts into the lesion, whereas BM gel and gelatin porous sponge did not. We conclude that the transplantation of LN-rich porous sponge promotes neuroblast migration into cortical lesions. This study highlights the possibility of using artificial blood vessel scaffolds to promote the regeneration of injured cerebral cortex.
脑损伤后，由内源性神经干细胞产生的神经母细胞利用血管作为支架向损伤部位迁移，这增加了重建血管网络支架作为促进内源性神经元再生策略的可能性。在本研究中，我们基于血管支架的成分和形态设计了生物材料，并检测了它们引导神经母细胞迁移到小鼠脑损伤部位的能力。移植含有基底膜（BM）基质成分的多孔海绵可增强神经母细胞向损伤部位的迁移，详细的形态学检查表明浸润细胞将BM海绵用作迁移支架。富含层粘连蛋白（LN）的多孔海绵也增强了神经母细胞向损伤部位的迁移，而BM凝胶和明胶多孔海绵则没有。我们得出结论，富含LN的多孔海绵移植可促进神经母细胞向皮质损伤部位的迁移。本研究突出了使用人工血管支架促进损伤大脑皮质再生的可能性。
