Saikh K U, Mitra S, Bhattacharyya F K
Indian Institute of Chemical Biology, Calcutta.
J Clin Microbiol. 1989 May;27(5):1090-4. doi: 10.1128/jcm.27.5.1090-1094.1989.
The effects of amino acid supplementation of commercially available semidefined GC media (Oxoid GC medium and Difco GC base) on the stability of the virulent colony phenotype of Neisseria gonorrhoeae were studied. The -SH-containing amino acids, together with glycine, were found to cause segregation of avirulent colony types followed by growth inhibition after addition of 40 mg/100 ml. Proline and arginine were found to change the colony phenotype profile significantly without reducing the viable count. Arginine supplementation in both media caused a progressive loss of virulent colony types, whereas proline had the opposite effect.
研究了在市售半限定GC培养基（Oxoid GC培养基和Difco GC基础培养基）中添加氨基酸对淋病奈瑟菌强毒株落表型稳定性的影响。发现含-SH的氨基酸与甘氨酸一起，在添加40mg/100ml后会导致无毒菌落类型的分离，随后生长受到抑制。发现脯氨酸和精氨酸可显著改变菌落表型谱，但不降低活菌数。在两种培养基中添加精氨酸都会导致强毒株落类型逐渐减少，而脯氨酸则有相反的效果。
