Hendry A T
Can J Microbiol. 1983 Oct;29(10):1309-13. doi: 10.1139/m83-204.
Neisseria gonorrhoeae strains can be grouped or differentiated (auxotyped) by their requirements for none, or any one or more of proline, uracil, hypoxanthine, and citrulline or ornithine. Most strains were readily assessed because they responded with growth or no growth on each defined auxanographic medium. Other strains gave indeterminate responses on agar and the reasons were not obvious. Liquid growth studies for quantifying the usual responses showed that yields of appropriate N. gonorrhoeae auxotrophs were proportional to replacement concentrations of any one of these amino acids or bases, of methionine, or of cysteine plus cystine. This type of response, where log growth rates and lag times were unaffected, is proposed as the basis for defining (simple) auxotrophy in gonococci. The formula of the defined medium was improved by increasing proline, uracil, and hypoxanthine beyond limiting concentrations, and decreasing citrulline or ornithine, and cysteine plus cystine. Fatty acid--free bovine albumin was used to ensure homogeneous growth in liquid media. In agar, it was superior to starch for the nonnutritive protective effect required by many strains.
淋病奈瑟菌菌株可根据其对脯氨酸、尿嘧啶、次黄嘌呤、瓜氨酸或鸟氨酸的需求情况进行分组或鉴别（确定其营养缺陷型），这些需求可以是不需要任何一种，也可以是需要其中任何一种或多种。大多数菌株很容易评估，因为它们在每种特定的营养缺陷型鉴别培养基上要么生长，要么不生长。其他菌株在琼脂上的反应不确定，原因不明。用于量化常见反应的液体生长研究表明，合适的淋病奈瑟菌营养缺陷型菌株的产量与这些氨基酸或碱基中的任何一种、甲硫氨酸、或半胱氨酸加胱氨酸的替代浓度成正比。这种对数生长速率和延迟时间不受影响的反应类型，被提议作为定义淋球菌（简单）营养缺陷型的基础。通过将脯氨酸、尿嘧啶和次黄嘌呤的浓度提高到极限浓度以上，并降低瓜氨酸或鸟氨酸以及半胱氨酸加胱氨酸的浓度，改进了特定培养基的配方。无脂肪酸牛血清白蛋白用于确保液体培养基中的均匀生长。在琼脂中，对于许多菌株所需的非营养性保护作用，它比淀粉更优越。
