利用吉姆萨染色和着丝粒特异性荧光原位杂交进行双着丝粒染色体畸变分析用于生物剂量测定：印度各实验室间及实验室内部的比较
Dicentric chromosome aberration analysis using giemsa and centromere specific fluorescence in-situ hybridization for biological dosimetry: An inter- and intra-laboratory comparison in Indian laboratories.
作者信息
Bhavani M, Tamizh Selvan G, Kaur Harpreet, Adhikari J S, Vijayalakshmi J, Venkatachalam P, Chaudhury N K
机构信息
Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India.
Department of Human Genetics, College of Biomedical Sciences Technology and Research, Sri Ramachandra University, Porur, Chennai 600116, Tamil Nadu, India; Chemical Radioprotector and Radiation Dosimetry Research Group, Institute of Nuclear Medicine and Allied Sciences, Brig Mazumdar Road, DRDO, Timarpur, New Delhi 110054, India.
出版信息
Appl Radiat Isot. 2014 Sep;92:85-90. doi: 10.1016/j.apradiso.2014.06.004. Epub 2014 Jun 15.
To facilitate efficient handling of large samples, an attempt towards networking of laboratories in India for biological dosimetry was carried out. Human peripheral blood samples were exposed to (60)Co γ-radiation for ten different doses (0-5Gy) at a dose rate of 0.7 and 2Gy/min. The chromosomal aberrations (CA) were scored in Giemsa-stained and fluorescence in-situ hybridization with centromere-specific probes. No significant difference (p>0.05) was observed in the CA yield for given doses except 4 and 5Gy, between the laboratories, among the scorers and also staining methods adapted suggest the reliability and validates the inter-lab comparisons exercise for triage applications.
为便于高效处理大量样本，印度开展了一项关于生物剂量测定实验室联网的尝试。将人类外周血样本以0.7和2Gy/分钟的剂量率暴露于（60）Coγ辐射下，设置十种不同剂量（0 - 5Gy）。在吉姆萨染色以及使用着丝粒特异性探针进行荧光原位杂交后，对染色体畸变（CA）进行评分。除4Gy和5Gy剂量外，各实验室之间、评分者之间以及所采用的染色方法之间，在给定剂量下的CA产率均未观察到显著差异（p>0.05），这表明其可靠性并验证了用于分流应用的实验室间比较工作。
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