Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Int J Syst Evol Microbiol. 2014 Oct;64(Pt 10):3422-3426. doi: 10.1099/ijs.0.063297-0. Epub 2014 Jul 11.
A Gram-stain-negative, cream-pigmented, motile, extremely halophilic archaeon, designated strain IC38(T), was isolated from a saline mud sample taken from a hypersaline lake, Aran-Bidgol, in Iran. The strain required at least 2.5 M NaCl for growth. However, MgCl2 was not required. Optimal growth occurred with 4.3 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 35 °C, respectively, and strain IC38(T) was able to grow over a pH range of 6.5-9.0, and a temperature range of 25-45 °C. Analysis of the 16S rRNA gene sequence revealed that strain IC38(T) clustered with the two species of the genus Halovivax, Halovivax asiaticus EJ-46(T) and Halovivax ruber XH-70(T), with sequence similarities of 96.4% and 96.1%, respectively. The similarities between the rpoB' gene of the novel strain and Halovivax asiaticus and Halovivax ruber were 90.7% and 90.3%, respectively. The polar lipid pattern of strain IC38(T) consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Three unidentified glycolipids and two minor phospholipids were also observed. The DNA G+C content of strain IC38(T) was 62.6 mol%. On the basis of the phylogenetic analysis, as well as the biochemical and physiological characteristics, the new isolate is suggested to be a representative of a novel species of the genus Halovivax, for which the name Halovivax limisalsi sp. nov. is proposed. The type strain of Halovivax limisalsi is IC38(T) ( = IBRC-M 10022(T) = KCTC 4051(T)).
一株革兰氏阴性、奶油色、运动、极端嗜盐古菌，命名为 IC38(T) 菌株，从伊朗高盐湖 Aran-Bidgol 的盐泥样本中分离得到。该菌株的生长需要至少 2.5 M NaCl，但不需要 MgCl2。在 4.3 M NaCl 和 0.2 M MgCl2 下生长最佳。最佳生长的 pH 和温度分别为 pH 7.0 和 35°C，IC38(T) 菌株能够在 pH 6.5-9.0 和温度 25-45°C 的范围内生长。16S rRNA 基因序列分析表明，菌株 IC38(T)与 Halovivax 属的两个种，即 Halovivax asiaticus EJ-46(T)和 Halovivax ruber XH-70(T)聚在一起，序列相似性分别为 96.4%和 96.1%。新型菌株与 Halovivax asiaticus 和 Halovivax ruber 的 rpoB'基因相似度分别为 90.7%和 90.3%。菌株 IC38(T)的极性脂模式由磷脂酰甘油和磷脂酰甘油磷酸甲酯组成。还观察到三种未鉴定的糖脂和两种次要磷脂。菌株 IC38(T)的 DNA G+C 含量为 62.6 mol%。基于系统发育分析以及生化和生理特性，新分离株被建议代表 Halovivax 属的一个新种，命名为 Halovivax limisalsi sp. nov.。Halovivax limisalsi 的模式菌株为 IC38(T)（=IBRCM 10022(T)=KCTC 4051(T)）。
