Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
Extremophile Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1331-1336. doi: 10.1099/ijs.0.031419-0. Epub 2011 Jul 29.
A novel red-pigmented halophilic archaeon, strain EB27(T), was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain EB27(T) were non-motile and pleomorphic (rods to triangular or disc-shaped). Strain EB27(T) required at least 2.5 M NaCl and 0.1 M MgCl(2) for growth. Optimal growth was achieved at 4 M NaCl and 0.5 M MgCl(2). The optimum pH and temperature for growth were pH 7.5 and 40 °C; it was able to grow at pH 6.0-8.0 and 25-50 °C. 16S rRNA gene sequence analysis showed that strain EB27(T) is a member of the family Halobacteriaceae; however, levels of 16S rRNA gene sequence similarity were as low as 90.0, 89.3 and 89.1 % to the most closely related haloarchaeal taxa, namely Halalkalicoccus tibetensis DS12(T), Halosimplex carlsbadense 2-9-1(T) and Halorhabdus utahensis AX-2(T), respectively. The DNA G+C content of strain EB27(T) was 61 mol%. Strain EB27(T) contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, together with two minor phospholipids. The only quinone present was MK-8(II-H(2)). Physiological, biochemical and phylogenetic differences between strain EB27(T) and recognized genera of extremely halophilic archaea suggest that this strain represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halovenus aranensis gen. nov., sp. nov. is proposed. The type strain of Halovenus aranensis, the type species of the new genus, is strain EB27(T) ( = IBRC-M 10015(T) = CGMCC 1.11001(T)).
一株新型红色嗜盐古菌，EB27(T) 菌株，从伊朗高盐度干盐湖 Aran-Bidgol 中分离得到。EB27(T) 菌株的细胞是非运动的，多形的（杆状到三角形或盘状）。EB27(T) 菌株的生长至少需要 2.5 M NaCl 和 0.1 M MgCl2。在 4 M NaCl 和 0.5 M MgCl2 下达到最佳生长。最佳生长的 pH 和温度为 pH7.5 和 40°C；它可以在 pH6.0-8.0 和 25-50°C 下生长。16S rRNA 基因序列分析表明，EB27(T) 菌株是盐杆菌科的成员；然而，16S rRNA 基因序列相似性水平与最接近的嗜盐古菌分类群低至 90.0%、89.3%和 89.1%，分别为 Halalkalicoccus tibetensis DS12(T)、Halosimplex carlsbadense 2-9-1(T) 和 Halorhabdus utahensis AX-2(T)。EB27(T) 菌株的 DNA G+C 含量为 61mol%。EB27(T) 菌株含有磷脂酰甘油和磷脂酰甘油磷酸甲基酯，这是在嗜盐古菌中发现的常见磷脂，同时还含有两种少量的磷脂。唯一存在的醌是 MK-8(II-H2)。EB27(T) 菌株与已识别的极端嗜盐古菌属之间的生理、生化和系统发育差异表明，该菌株代表了盐杆菌科中的一个新属的新种，建议将其命名为 Halovenus aranensis 属。新属的模式种 Halovenus aranensis 的模式菌株是 EB27(T) 菌株（=IBRCM10015(T)=CGMCC1.11001(T)）。
