Ura Blendi, Feriotto Giordana, Monasta Lorenzo, Bilel Sabrine, Zweyer Marina, Celeghini Claudio
Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy.
Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
Taiwan J Obstet Gynecol. 2014 Jun;53(2):232-4. doi: 10.1016/j.tjog.2014.03.001.
To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24).
Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction.
By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE.
Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
鉴定妊娠早期（12 - 14周）孕妇血清中差异表达的微小RNA（miRNA），这些孕妇在妊娠晚期（孕24周）发生了重度子痫前期（sPE），并与正常妊娠孕妇（n = 24）进行比较。
对妊娠12 - 14周全血的血清进行TaqMan低密度阵列芯片（人类miRNA芯片V3.0）微阵列分析，并进行定量实时聚合酶链反应。
通过使用TaqMan低密度阵列芯片技术，与正常女性相比，19种成熟miRNA在后来发生sPE的女性组中出现差异表达。通过定量实时聚合酶链反应分析验证了4种miRNA（miR - 1233、miR - 520、miR - 210、miR - 144）的表达。MiR - 1233在后来发生sPE的女性血清中表达上调最为明显。
循环miRNA值得进一步研究，以探索它们在子痫前期发病机制中的潜在作用。特别是，miR -
