Yuan Wei, Sui Chenguang, Liu Qian, Tang Wanyan, An Huaying, Ma Jie
State Key Laboratory of Molecular Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Department of Abdominal Surgical Oncology, Cancer Institute & Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
PLoS One. 2014 Jul 14;9(7):e102017. doi: 10.1371/journal.pone.0102017. eCollection 2014.
Metastasis is the main cause of mortality in patients with solid tumours. Identifying the exact molecules associated with CRC metastasis may be crucial to understand the process, which might also be translated to the diagnosis and treatment of CRC. In this study, we investigate the association of microRNA expression patterns with the lymph node metastasis of colorectal cancer. Among these candidate miRNAs, the expression of miRNA-145 was significantly related to lymph node metastasis of CRC. Both in vitro and in vivo study demonstrated that up-regulation of miR-145 could improve the ability of migration and invasion of colorectal cancer cell, while no effect on proliferation was observed. The mechanism of this promotion is associated with the stabilization of Hsp-27, a protein which plays an important role in the promotion of metastasis. These results may be crucial to understanding CRC metastasis and may be translated to the diagnosis and treatment of CRC.
转移是实体瘤患者死亡的主要原因。确定与结直肠癌转移相关的精确分子对于理解这一过程可能至关重要，这也可能转化为结直肠癌的诊断和治疗。在本研究中，我们调查了微小RNA表达模式与结直肠癌淋巴结转移的关联。在这些候选微小RNA中，miRNA-145的表达与结直肠癌的淋巴结转移显著相关。体外和体内研究均表明，miR-145的上调可提高结肠癌细胞的迁移和侵袭能力，而未观察到对增殖的影响。这种促进作用的机制与热休克蛋白27（Hsp-27）的稳定有关，Hsp-27是一种在转移促进中起重要作用的蛋白质。这些结果对于理解结直肠癌转移可能至关重要，并且可能转化为结直肠癌的诊断和治疗。
