Liu Qing-Bo, Huang Xiao-Xiao, Bai Ming, Chang Xiao-Bing, Yan Xin-Jia, Zhu Tao, Zhao Wei, Peng Ying, Song Shao-Jiang
School of Traditional Chinese Materia Medica, ‡Key Laboratory of Structure-Based Drug Design and Discovery (Ministry of Education), and #School of Pharmacy, Shenyang Pharmaceutical University , Shenyang 110016, People's Republic of China.
J Agric Food Chem. 2014 Aug 6;62(31):7796-803. doi: 10.1021/jf502171z. Epub 2014 Jul 24.
Prunus tomentosa seeds were researched for antioxidant and anti-inflammatory constituents. By activity-guided fractionation of P. tomentosa seed extract, six new dihydrobenzofuran neolignans, prunustosanans AI-IV (1-4) and prunustosanansides AI and AII (5 and 6), together with 10 known compounds (7-16) were isolated from bioactive fraction. The structures were determined by spectroscopic analyses, especially NMR, HRESIMS, and CD spectra. The antioxidant activity was greatest for 5, 10, and 12 against DPPH radical and for 8, 9, and 13 against ABTS radical. Moreover, compounds 7 and 11 exhibited much stronger inhibitory activity on nitric oxide (NO) production in murine microglia BV-2 compared with positive control minocycline (IC50 = 19.7 ± 1.5 μM). The results show that P. tomentosa seeds can be regarded as a potential source of antioxidants and inflammation inhibitors.
对毛樱桃种子的抗氧化和抗炎成分进行了研究。通过对毛樱桃种子提取物进行活性导向分离，从生物活性部分分离出6种新的二氢苯并呋喃新木脂素，即毛樱桃素AI-IV（1-4）和毛樱桃素苷AI和AII（5和6），以及10种已知化合物（7-16）。通过光谱分析，尤其是核磁共振（NMR）、高分辨电喷雾电离质谱（HRESIMS）和圆二色（CD）光谱确定了其结构。化合物5、10和12对DPPH自由基的抗氧化活性最强，化合物8、9和13对ABTS自由基的抗氧化活性最强。此外，与阳性对照米诺环素（IC50 = 19.7 ± 1.5 μM）相比，化合物7和11对小鼠小胶质细胞BV-2中一氧化氮（NO）的产生表现出更强的抑制活性。结果表明，毛樱桃种子可被视为抗氧化剂和炎症抑制剂的潜在来源。
