Qi Yutong, Choi Se-In, Son So-Ri, Han Hee-Soo, Ahn Hye Shin, Shin Yu-Kyong, Lee Sun Hee, Lee Kyung-Tae, Kwon Hak Cheol, Jang Dae Sik
Department of Life and Nanopharmaceutical Sciences, Graduate School Kyung Hee University, Seoul 02447, Korea.
Department of New Material Development, COSMAX BIO, Seongnam 13486, Korea.
Plants (Basel). 2020 Sep 18;9(9):1232. doi: 10.3390/plants9091232.
Nakai (Campanulaceae; Korean bellflower) is one of the endemic herbs of Korea. The plant has been used as traditional medicines for treating asthma, tonsillitis, and sore throat in Korea. A hot water extract of the leaves of exhibited a significant inhibitory effect on lipopolysaccharide (LPS)-stimulated prostaglandin E (PGE) production. Repetitive chromatographic separation of the hot water extract led to the isolation of three new neolignan glucosides, campanulalignans A-C (-), with 15 known compounds (-). The structures of new compounds - were elucidated by analyzing nuclear magnetic resonance (NMR) spectroscopic data, along with high resolution quadrupole time of flight mass (HR-Q-TOF-MS) spectrometric data. Among the isolates, simplidin (), 5-hydroxyconiferaldehyde (), icariside F (), benzyl--l-arabinopyranosyl-(1″→6')--d-glucopyranoside (), and kaempferol 3---d-apiosyl (1→2)--d-glucopyranoside () were isolated from the Campanulaceae family for the first time. The isolates (, , and -) were assessed for their anti-inflammatory effects on LPS-stimulated PGE production on RAW 264.7 cells. 7,8-Dihydrodehydrodiconiferyl alcohol (), 3',4--dimethylcedrusin 9---glucopyranoside (), pinoresinol di---d-glucoside (), ferulic acid (), 5-hydroxyconiferaldehyde (), and quercetin () showed significant inhibitory effects on LPS-stimulated PGE production.
紫斑风铃草（桔梗科；朝鲜风铃草）是韩国的本土草本植物之一。在韩国，该植物一直被用作治疗哮喘、扁桃体炎和喉咙痛的传统药物。紫斑风铃草叶的热水提取物对脂多糖（LPS）刺激的前列腺素E（PGE）生成具有显著抑制作用。对热水提取物进行反复色谱分离，得到了三种新的新木脂素糖苷，风铃草新木脂素A - C（-），以及15种已知化合物（-）。通过分析核磁共振（NMR）光谱数据以及高分辨率四极杆飞行时间质谱（HR - Q - TOF - MS）数据，阐明了新化合物 - 的结构。在分离出的化合物中，辛普利定（）、5 - 羟基松柏醛（）、淫羊藿苷F（）、苄基 - β - L - 阿拉伯吡喃糖基 -（1″→6'）- β - D - 葡萄糖吡喃糖苷（）和山柰酚3 - β - D - 芹菜糖基（1→2）- β - D - 葡萄糖吡喃糖苷（）首次从桔梗科植物中分离得到。评估了分离出的化合物（、和 - ）对RAW 264.7细胞上LPS刺激的PGE生成的抗炎作用。7,8 - 二氢脱氢二松柏醇（）、3',4 - 二甲基雪松素9 - β - 葡萄糖苷（）、松脂醇二 - β - D - 葡萄糖苷（）、阿魏酸（）、5 - 羟基松柏醛（）和槲皮素（）对LPS刺激的PGE生成显示出显著抑制作用。
