Ito Yuma, Hirasawa Takashi, Shimizu Hiroshi
a Department of Bioinformatic Engineering, Graduate School of Information Science and Technology , Osaka University , Osaka , Japan.
Biosci Biotechnol Biochem. 2014;78(1):151-9. doi: 10.1080/09168451.2014.877816. Epub 2014 Apr 14.
We performed metabolic engineering on the budding yeast Saccharomyces cerevisiae for enhanced production of succinic acid. Aerobic succinic acid production in S. cerevisiae was achieved by disrupting the SDH1 and SDH2 genes, which encode the catalytic subunits of succinic acid dehydrogenase. Increased succinic acid production was achieved by eliminating the ethanol biosynthesis pathways. Metabolic profiling analysis revealed that succinic acid accumulated intracellularly following disruption of the SDH1 and SDH2 genes, which suggests that enhancing the export of intracellular succinic acid outside of cells increases succinic acid production in S. cerevisiae. The mae1 gene encoding the Schizosaccharomyces pombe malic acid transporter was introduced into S. cerevisiae, and as a result, succinic acid production was successfully improved. Metabolic profiling analysis is useful in producing chemicals for metabolic engineering of microorganisms.
我们对酿酒酵母进行了代谢工程改造，以提高琥珀酸的产量。通过破坏编码琥珀酸脱氢酶催化亚基的SDH1和SDH2基因，实现了酿酒酵母的需氧琥珀酸生产。通过消除乙醇生物合成途径，提高了琥珀酸产量。代谢谱分析表明，SDH1和SDH2基因破坏后，琥珀酸在细胞内积累，这表明增强细胞内琥珀酸向细胞外的输出可提高酿酒酵母中琥珀酸的产量。将编码粟酒裂殖酵母苹果酸转运蛋白的mae1基因导入酿酒酵母，结果成功提高了琥珀酸产量。代谢谱分析对于微生物代谢工程生产化学品很有用。
