de Smet M J, Friedman M B, Gunsalus I C
La Jolla Biological Laboratories, Salk Institute, San Diego, California 92138.
J Bacteriol. 1989 Sep;171(9):5155-61. doi: 10.1128/jb.171.9.5155-5161.1989.
Two Pseudomonas strains (PpG777 and PaG158) were derived from the parent isolate Pseudomonas incognita (putida). Strain PpG777 resembles the parental culture in growth on linalool as a source of carbon and slight growth on p-cymene, whereas PaG158 grows well on p-cymene, but not on linalool or other terpenes tested, and has a P. aeruginosa phenotype. Curing studies indicate that linalool metabolism is controlled by an extrachromosomal element whose loss forms a stable strain PaG158 with the p-cymene growth and P. aeruginosa phenotype characters. The plasmid can be transferred by PpG777 to both P. putida and P. aeruginosa strains. Surprisingly, the latter assume the P. putida phenotype. We conclude that the genetic potential to oxidize p-cymene is inherent in PpG777 but expression is repressed. Similarly, this observation implies that support of linalool oxidation effectively conceals the P. aeruginosa character.
两种假单胞菌菌株（PpG777和PaG158）源自亲本分离株未知假单胞菌（恶臭假单胞菌）。菌株PpG777在以芳樟醇为碳源的培养基上生长情况与亲本培养物相似，在对伞花烃上有轻微生长，而PaG158在对伞花烃上生长良好，但在芳樟醇或其他测试的萜类化合物上不生长，并且具有铜绿假单胞菌的表型。消除研究表明，芳樟醇代谢由一种染色体外元件控制，其缺失形成了具有对伞花烃生长和铜绿假单胞菌表型特征的稳定菌株PaG158。该质粒可由PpG777转移至恶臭假单胞菌和铜绿假单胞菌菌株。令人惊讶的是，后者呈现恶臭假单胞菌的表型。我们得出结论，氧化对伞花烃的遗传潜力在PpG777中是固有的，但表达受到抑制。同样，这一观察结果表明，芳樟醇氧化的支持有效地掩盖了铜绿假单胞菌的特征。
